Propagating and Banking Genetically Diverse Human Sapovirus Strains Using a Human Duodenal Cell Line: Investigating Antigenic Differences Between Strains

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2024 Aug 12

PMID 39132992

Authors

Tomoichiro Oka

Tian-Cheng Li

Kenzo Yonemitsu

Yasushi Ami

Yuriko Suzaki

Michiyo Kataoka

Yen Hai Doan

Yuko Okemoto-Nakamura

Takayuki Kobayashi

Hiroyuki Saito

Tetsuo Mita

Eisuke Tokuoka

Shinichiro Shibata

Tetsuya Yoshida

Hirotaka Takagi

Affiliations

Soon will be listed here.

Abstract

Importance: The control of human sapovirus, which causes acute gastroenteritis in individuals of all ages, is challenging because of its association with outbreaks similar to those caused by human norovirus. The establishment of conditions for efficient viral propagation of various viral strains is essential for understanding the infection mechanism and identifying potential control methods. In this study, two critical factors for human sapovirus propagation in a conventional human duodenal cell line were identified, and 15 strains of different genotypes that differed at the genetic and antigenic levels were isolated and used to prepare virus stocks. The preparation of virus stocks has not been successful for noroviruses, which belong to the same family as sapoviruses. Securing virus stocks of multiple human sapovirus strains represents a significant advance toward establishing a reliable experimental system that does not depend on limited virus-positive fecal material.

Citing Articles

Establishment of a Novel Caco-2-Based Cell Culture System for Human Sapovirus Propagation.

Fukuda Y, Ishikawa A, Ishiyama R, Takai-Todaka R, Haga K, Someya Y Genes Cells. 2025; 30(2):e70007.

PMID: 40001267 PMC: 11861566. DOI: 10.1111/gtc.70007.

References

Oka T, Doan Y, Shimoike T, Haga K, Takizawa T . First complete genome sequences of genogroup V, genotype 3 porcine sapoviruses: common 5'-terminal genomic feature of sapoviruses. Virus Genes. 2017; 53(6):848-855. DOI: 10.1007/s11262-017-1481-8. View

Harada S, Oka T, Tokuoka E, Kiyota N, Nishimura K, Shimada Y . A confirmation of sapovirus re-infection gastroenteritis cases with different genogroups and genetic shifts in the evolving sapovirus genotypes, 2002-2011. Arch Virol. 2012; 157(10):1999-2003. DOI: 10.1007/s00705-012-1387-7. View

Iizuka S, Takai-Todaka R, Ohshiro H, Kitajima M, Wang Q, Saif L . Detection of multiple human sapoviruses from imported frozen individual clams. Food Environ Virol. 2013; 5(2):119-25. DOI: 10.1007/s12560-013-9109-1. View

Hansman G, Guntapong R, Pongsuwanna Y, Natori K, Katayama K, Takeda N . Development of an antigen ELISA to detect sapovirus in clinical stool specimens. Arch Virol. 2005; 151(3):551-61. DOI: 10.1007/s00705-005-0630-x. View

Parwani A, Flynn W, Gadfield K, Saif L . Serial propagation of porcine enteric calicivirus in a continuous cell line. Effect of medium supplementation with intestinal contents or enzymes. Arch Virol. 1991; 120(1-2):115-22. DOI: 10.1007/BF01310954. View

Rivera-Gutierrez X, Moran P, Taboada B, Serrano-Vazquez A, Isa P, Rojas-Velazquez L . High Prevalence and Diversity of Caliciviruses in a Community Setting Determined by a Metagenomic Approach. Microbiol Spectr. 2022; 10(1):e0185321. PMC: 8865552. DOI: 10.1128/spectrum.01853-21. View

Madeley C, Cosgrove B . Letter: Caliciviruses in man. Lancet. 1976; 1(7952):199-200. DOI: 10.1016/s0140-6736(76)91309-x. View

Oka T, Mori K, Iritani N, Harada S, Ueki Y, Iizuka S . Human sapovirus classification based on complete capsid nucleotide sequences. Arch Virol. 2011; 157(2):349-52. DOI: 10.1007/s00705-011-1161-2. View

Zou L, Li Y, Zhou G, Huang Z, Ju C, Zhao C . A Large Acute Gastroenteritis Outbreak Associated with Both and Human Sapovirus - Beijing Municipality, China, 2021. China CDC Wkly. 2024; 5(52):1167-1173. PMC: 10757730. DOI: 10.46234/ccdcw2023.219. View

10.

Oka T, Miyashita K, Katayama K, Wakita T, Takeda N . Distinct genotype and antigenicity among genogroup II sapoviruses. Microbiol Immunol. 2009; 53(7):417-20. DOI: 10.1111/j.1348-0421.2009.00133.x. View

11.

Kauppinen A, Pitkanen T, Al-Hello H, Maunula L, Hokajarvi A, Rimhanen-Finne R . Two Drinking Water Outbreaks Caused by Wastewater Intrusion Including Sapovirus in Finland. Int J Environ Res Public Health. 2019; 16(22). PMC: 6888097. DOI: 10.3390/ijerph16224376. View

12.

Dawson P, Karpen S . Intestinal transport and metabolism of bile acids. J Lipid Res. 2014; 56(6):1085-99. PMC: 4442867. DOI: 10.1194/jlr.R054114. View

13.

Oka T, Doan Y, Haga K, Mori K, Ogawa T, Yamazaki A . Genetic Characterization of Rare Genotype GII.5 Sapovirus Strain Detected from a Suspected Food-Borne Gastroenteritis Outbreak among Adults in Japan in 2010. Jpn J Infect Dis. 2016; 70(2):223-224. DOI: 10.7883/yoken.JJID.2016.468. View

14.

Song K, Lin X, Liu Y, Ji F, Zhang L, Chen P . Detection of Human Sapoviruses in Sewage in China by Next Generation Sequencing. Food Environ Virol. 2021; 13(2):270-280. PMC: 7985922. DOI: 10.1007/s12560-021-09469-x. View

15.

Euller-Nicolas G, Le Mennec C, Schaeffer J, Zeng X, Ettayebi K, Atmar R . Human Sapovirus Replication in Human Intestinal Enteroids. J Virol. 2023; 97(4):e0038323. PMC: 10134857. DOI: 10.1128/jvi.00383-23. View

16.

Hansman G, Saito H, Shibata C, Ishizuka S, Oseto M, Oka T . Outbreak of gastroenteritis due to sapovirus. J Clin Microbiol. 2007; 45(4):1347-9. PMC: 1865854. DOI: 10.1128/JCM.01854-06. View

17.

Shirakawa D, Shirasaki N, Hu Q, Matsushita T, Matsui Y, Takagi H . Investigation of removal and inactivation efficiencies of human sapovirus in drinking water treatment processes by applying an in vitro cell-culture system. Water Res. 2023; 236:119951. DOI: 10.1016/j.watres.2023.119951. View

18.

Oka T, Yamamoto S, Iritani N, Sato S, Tatsumi C, Mita T . Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses. Arch Virol. 2020; 165(10):2335-2340. PMC: 7383071. DOI: 10.1007/s00705-020-04746-9. View

19.

Hansman G, Oka T, Sakon N, Takeda N . Antigenic diversity of human sapoviruses. Emerg Infect Dis. 2008; 13(10):1519-25. PMC: 2851512. DOI: 10.3201/eid1310.070402. View

20.

Oka T, Iritani N, Okada M, Ogawa T, Iizuka S, Tatsumi C . First Complete Genome Sequences of Human Sapovirus Strains Classified as GI.3, GI.4, GI.6, GI.7, and GII.7. Genome Announc. 2018; 6(12). PMC: 5864949. DOI: 10.1128/genomeA.00168-18. View