Enhanced Repellent and Anti-nutritional Activities of Polymeric Nanoparticles Containing Essential Oils Against Red Flour Beetle, Tribolium Castaneum

Overview

Journal Sci Rep

Specialty Science

Date 2024 Aug 10

PMID 39127742

Authors

Fatemeh Khandehroo

Gholamhossein Moravvej

Nafiseh Farhadian

Hossein Ahmadzadeh

Affiliations

Soon will be listed here.

Abstract

Encapsulation of essential oils (EOs) is an important strategy that can be applied to intensify the stability and efficiency of these compounds in integrated pest management. The present study aimed to investigate the sub-lethal activity of polymer-based EOs nanoparticles against red flour beetle, Tribolium castaneum adults as an important critical pest of stored products. Chitosan nanoparticles (CSNPs) containing garlic and cinnamon essential oils (GEO and CEO) prepared using the ionic cross-link technique. Stability of nano-formulations evaluated over temperature and storage time. The fumigant effect (LC, LC, LC) and contact toxicity (LC, LC, LC) determined. In addition, the contact toxicities of EOs and their nanoparticles on nutritional indices evaluated. An olfactometer used to assess the repellent activity of EOs and EOs loaded in CSNPs (EOs@CSNPs) in sub-lethal fumigant concentrations. Characterization results showed GEO loaded in CSNPs has particle size of 231.14 ± 7.55 nm, polydispersity index (PDI) value of 0.15 ± 0.02, encapsulation efficiency (EE) percentage of 76.77 ± 0.20 and zeta potential of - 18.82 ± 0.90 mV, in which these values for the CEO loaded in CSNPs (CEO@CSNPs) changed to 303.46 ± 0.00 nm, 0.20 ± 0.05, 86.81 ± 0.00% and - 20.16 ± 0.35 mV, respectively. A lower PDI value for both CSNPs showed an appropriate NPs size distribution. Furthermore, NPs size and encapsulation efficiency did not change in various temperatures and during four months which confirm good stability of the EOs@CSNPs. In LC of GEO@CSNPs, the maximum repellency was determined as 66.66 ± 3.33. Among nutritional indices, in LC of GEO@CSNPs, the relative growth rate (RGR) (0.011 ± 0.003 mg.mg.day), relative consumption rate (RCR) (0.075 ± 0.004 mg.mg.day) and feeding deterrence index (FDI) (54.662 ± 1.616%) were more affected, so GEO@CSNPs was more effective than CEO@CSNPs. The results of repellent and anti-dietary activities of EOs and EOs@CSNPs confirmed the higher repellency and adverse effectivity on nutritional indices of Tribolium castaneum pest treated with EOs@CSNPs compared to free EOs. In conclusion, the NPs form of GEO and CEO can be a novel and efficient carrier for improving the repellent and anti-nutritional activities of EOs.

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References

Makimori R, Endo E, Makimori J, Zanqueta E, Ueda-Nakamura T, Leimann F . Preparation, characterization and antidermatophytic activity of free- and microencapsulated cinnamon essential oil. J Mycol Med. 2020; 30(2):100933. DOI: 10.1016/j.mycmed.2020.100933. View

Alkhaibari A, Alanazi A . Chemical Composition and Insecticidal, Antiplasmodial, and Anti-Leishmanial Activity of Essential Oil and Its Main Constituents. Evid Based Complement Alternat Med. 2022; 2022:6371274. PMC: 8825289. DOI: 10.1155/2022/6371274. View

Plata-Rueda A, Martinez L, dos Santos M, Fernandes F, Wilcken C, Soares M . Insecticidal activity of garlic essential oil and their constituents against the mealworm beetle, Tenebrio molitor Linnaeus (Coleoptera: Tenebrionidae). Sci Rep. 2017; 7:46406. PMC: 5397855. DOI: 10.1038/srep46406. View

Duarte S, Magro A, Tomas J, Hilario C, Alvito P, Ferreira R . The Interaction between and Mycotoxigenic in Maize Flour. Insects. 2021; 12(8). PMC: 8396807. DOI: 10.3390/insects12080730. View

Dunkel F . The relationship of insects to the deterioration of stored grain by fungi. Int J Food Microbiol. 1988; 7(3):227-44. DOI: 10.1016/0168-1605(88)90042-6. View

Masarudin M, Cutts S, Evison B, Phillips D, Pigram P . Factors determining the stability, size distribution, and cellular accumulation of small, monodisperse chitosan nanoparticles as candidate vectors for anticancer drug delivery: application to the passive encapsulation of [(14)C]-doxorubicin. Nanotechnol Sci Appl. 2015; 8:67-80. PMC: 4686320. DOI: 10.2147/NSA.S91785. View

Gonzalez J, Yeguerman C, Marcovecchio D, Delrieux C, Ferrero A, Fernandez Band B . Evaluation of sublethal effects of polymer-based essential oils nanoformulation on the german cockroach. Ecotoxicol Environ Saf. 2016; 130:11-8. DOI: 10.1016/j.ecoenv.2016.03.045. View

Yang F, Li X, Zhu F, Lei C . Structural characterization of nanoparticles loaded with garlic essential oil and their insecticidal activity against Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). J Agric Food Chem. 2009; 57(21):10156-62. DOI: 10.1021/jf9023118. View

Yun T, Park S, Yu J, Hwang Y, Hong K . Isolation and Identification of Fungal Species from the Insect Pest in Rice Processing Complexes in Korea. Plant Pathol J. 2018; 34(5):356-366. PMC: 6200038. DOI: 10.5423/PPJ.OA.02.2018.0027. View

10.

Pitasawat B, Choochote W, Tuetun B, Tippawangkosol P, Kanjanapothi D, Jitpakdi A . Repellency of aromatic turmeric Curcuma aromatica under laboratory and field conditions. J Vector Ecol. 2004; 28(2):234-40. View

11.

Kah M, Hofmann T . Nanopesticide research: current trends and future priorities. Environ Int. 2013; 63:224-35. DOI: 10.1016/j.envint.2013.11.015. View

12.

Trongtokit Y, Rongsriyam Y, Komalamisra N, Apiwathnasorn C . Comparative repellency of 38 essential oils against mosquito bites. Phytother Res. 2005; 19(4):303-9. DOI: 10.1002/ptr.1637. View

13.

Yuan Y, Huang M, Pang Y, Yu F, Chen C, Liu L . Variations in Essential Oil Yield, Composition, and Antioxidant Activity of Different Plant Organs from Blumea balsamifera (L.) DC. at Different Growth Times. Molecules. 2016; 21(8). PMC: 6273516. DOI: 10.3390/molecules21081024. View

14.

Nerio L, Olivero-Verbel J, Stashenko E . Repellent activity of essential oils: a review. Bioresour Technol. 2009; 101(1):372-8. DOI: 10.1016/j.biortech.2009.07.048. View

15.

Herrera-Calderon O, Chacaltana-Ramos L, Huayanca-Gutierrez I, Algarni M, Alqarni M, El-Saber Batiha G . Chemical Constituents, In Vitro Antioxidant Activity and In Silico Study on NADPH Oxidase of L. (Garlic) Essential Oil. Antioxidants (Basel). 2021; 10(11). PMC: 8615010. DOI: 10.3390/antiox10111844. View

16.

Nel A, Madler L, Velegol D, Xia T, Hoek E, Somasundaran P . Understanding biophysicochemical interactions at the nano-bio interface. Nat Mater. 2009; 8(7):543-57. DOI: 10.1038/nmat2442. View

17.

Othman N, Masarudin M, Yee Kuen C, Dasuan N, Abdullah L, Md Jamil S . Synthesis and Optimization of Chitosan Nanoparticles Loaded with L-Ascorbic Acid and Thymoquinone. Nanomaterials (Basel). 2018; 8(11). PMC: 6267081. DOI: 10.3390/nano8110920. View

18.

Jamil B, Abbasi R, Abbasi S, Khan S, Ihsan A, Javed S . Encapsulation of Cardamom Essential Oil in Chitosan Nano-composites: Efficacy on Antibiotic-Resistant Bacterial Pathogens and Cytotoxicity Studies. Front Microbiol. 2016; 7:1580. PMC: 5048087. DOI: 10.3389/fmicb.2016.01580. View

19.

Yezerski A, Ciccone C, Rozitski J, Volingavage B . The effects of a naturally produced benzoquinone on microbes common to flour. J Chem Ecol. 2007; 33(6):1217-25. DOI: 10.1007/s10886-007-9293-2. View

20.

Ebadollahi A, Naseri B, Abedi Z, Setzer W, Changbunjong T . Promising Insecticidal Efficiency of Essential Oils Isolated from Four Cultivated Species in Iran against the Lesser Grain Borer, (F.). Insects. 2022; 13(6). PMC: 9224839. DOI: 10.3390/insects13060517. View