Genome-Wide Analysis and Expression Profiling of Lectin Receptor-like Kinase Genes in Watermelon ()

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Aug 10

PMID 39125826

Authors

Duo Lv

Gang Wang

Jiaqi You

Lihua Zhu

Hongjuan Yang

Biting Cao

Weihong Gu

ChaoHan Li

Affiliations

Soon will be listed here.

Abstract

Watermelon is one of the most important edible plants worldwide. Owing to its special cultivation conditions, watermelon is exposed to many biological and abiotic stresses during its development. Lectin receptor-like kinases (LecRLKs) are plant-specific membrane proteins that play important roles in sensing and responding to environmental stimuli. Although the gene family has been identified in a variety of plants, a comprehensive analysis has not yet been undertaken in watermelon. In this study, 61 putative genes were identified in watermelon, consisting of 36 G-type, 24 L-type, and 1 C-type genes. They were distributed in clusters on chromosomes, and members from the same subfamily were mostly clustered together. The analysis of the phylogenetic tree and conserved motif indicated that there were obvious differences among three ClaLecRLK subfamilies, and there was also rich diversity in the C-terminal within subfamilies. A collinear analysis revealed that the evolution of the gene family in different Cucurbitaceae crops was asynchronous. Furthermore, the analysis of the ClaLecRLK protein structure showed that not all proteins contained signal peptides and a single transmembrane domain. A subcellular localization assay confirmed that the number and position of transmembrane domains did not affect ClaLecRLK protein localization in cells. Transcriptome data revealed distinct expression patterns of genes of watermelon in various tissues, and their responses to different fungi infection were also significantly different. Finally, the potential binding sites of the genes targeted by miRNA were predicted. This study enhances the understanding of the characteristics and functions of the gene family in watermelon and opens up the possibility of exploring the roles that genes may play in the life cycle of Cucurbitaceae plants.

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