Exploring the Antimicrobial and Antioxidant Properties of Strain E25-2 Isolated from Moroccan Forest Soil

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 Aug 6

PMID 39104582

Authors

Said Rammali

Alin Ciobica

Mohamed El Aalaoui

Abdellatif Rahim

Fatima Zahra Kamal

Khadija Dari

Abdelkrim Khattabi

Laura Romila

Bogdan Novac

Antoneta Petroaie

Bouchaib Bencharki

Affiliations

Soon will be listed here.

Abstract

The alarming rise in antimicrobial resistance (AMR) has created a significant public health challenge, necessitating the discovery of new therapeutic agents to combat infectious diseases and oxidative stress-related disorders. The strain E25-2, isolated from Moroccan forest soil, represents a potential avenue for such research. This study aimed to identify the isolate E25-2, obtained from soil in a cold Moroccan ecosystem, and further investigate its antimicrobial and antioxidant activities. Phylogenetic analysis based on 16S rRNA gene sequences revealed the strain's classification within the genus, with a sequence closely resembling that of AS4.0578 (96.10% similarity). Antimicrobial activity in solid media showed moderate to strong activity against ATCC 25923, strain ATCC 14579, strain ATCC 25922, strain ATCC 60193 and 4 phytopathogenic fungi. In addition, ethyl acetate extract of this isolate demonstrated potent antimicrobial activity against 7 clinically multi-drug resistant bacteria. Furthermore, it demonstrated antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals, as well as a significant increase in ferric reducing antioxidant power. A significant positive correlation was observed between antioxidant activities and total content of phenolic compounds ( < 0.0001), along with flavonoids ( < 0.0001). Furthermore, gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of amines, hydroxyl groups, pyridopyrazinone rings, esters and pyrrolopyrazines. The genus could offer promising prospects in the fight against antibiotic resistance and in the prevention against oxidative stress related diseases.

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