The Establishment and Severity Assessment of Ultrasound-guided Prenatal Bronchopulmonary Dysplasia Model in Rat

Overview

Journal Sci Rep

Specialty Science

Date 2024 Aug 5

PMID 39103397

Authors

Heng-Jie Shi

Shi-Jie Zhang

Fang-Ping Lai

Jiang-Feng Dai

Jia-Wen Li

Wu Xu

Guo-Rong Lyu

Shao-Zheng He

Affiliations

Soon will be listed here.

Abstract

To study a new method for establishing animal models of prenatal bronchopulmonary dysplasia (BPD), we used lung ultrasound score (LUS) to semi-quantitatively assess the severity of lung lesions in model rats. Lipopolysaccharide (LPS) was injected into the right lung of the fetus of the rat under ultrasound-guided, and the right lung of the neonates were scanning for LUS. Specimens were collected for pathological scoring and detection of pulmonary surfactant-associated glycoprotein (SP)-C and vascular endothelial growth factor (VEGF) expression quantity. The correlation between LUS and pathological scores was analyzed. (1) The animal models were consistent with the pathological manifestations of BPD. (2) It showed a strong positive correlation between LUS and pathological scores in animal models (r = 0.84, P < 0.005), and the expression quantity of SP-C and VEGF in lung tissue were decreased (both P < 0.05). Animal models established by ultrasound-guided puncture of the lung of rats and injection of LPS were consistent with the manifestation of BPD. This method could be used to establish animal models of BPD before birth, and the severity of BPD could be assessed by using LUS.

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