Growth Inhibitory Effect of (manuka) Chloroform Extract on Breast and Liver Cancer Cell Lines

Overview

Journal J Adv Vet Anim Res

Specialty Veterinary Medicine

Date 2024 Aug 5

PMID 39101096

Authors

Mohammed Al-Zharani

Affiliations

Soon will be listed here.

Abstract

Objective: Research has demonstrated that possesses various therapeutic benefits. This study set out to determine whether or not extracts had any effect on the ability of HepG2 and MCF-7 breast cancer cells to survive.

Materials And Methods: The antiproliferative activity of extracts was explored using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays. The most active fraction was selected to investigate its effects on apoptosis induction using flow cytometry and quantitative real-time polymerase chain reaction. The constituents of this fraction were characterized using GC-MS analysis.

Results: Research demonstrated that the chloroform fraction of (LSCF) significantly impacted the HepG2 and MCF-7 cancer cell lines. Treatment with LSCF led to a notable rise in both early and late apoptotic cells. Furthermore, there was an upregulation in the mRNA levels of P53, Bax, and caspases, while the expression of Bcl-2 mRNA saw a decrease. The analysis of LSCF revealed the primary components to be cis-calamenene, beta-eudesmol, cyclododecane, and alpha-muurolene.

Conclusion: The study showed the promising antiproliferative activity of , suggesting its potential application for cancer treatment.

Citing Articles

Manuka Essential Oil Triggers Apoptosis and Activation of c-Jun N-Terminal Kinase in Fibroblasts and Fibrosarcoma Cells.

Bass N, Parekh M, Satyal P, Soni S, Jacob J, Mack J Molecules. 2024; 29(21).

PMID: 39519810 PMC: 11547341. DOI: 10.3390/molecules29215168.

References

Mamdouh A, Khodeer D, Tantawy M, Moustafa Y . In-vitro and in-vivo investigation of amygdalin, metformin, and combination of both against doxorubicin on hepatocellular carcinoma. Life Sci. 2021; 285:119961. DOI: 10.1016/j.lfs.2021.119961. View

Kari S, Subramanian K, Altomonte I, Murugesan A, Yli-Harja O, Kandhavelu M . Programmed cell death detection methods: a systematic review and a categorical comparison. Apoptosis. 2022; 27(7-8):482-508. PMC: 9308588. DOI: 10.1007/s10495-022-01735-y. View

Hashem S, Ali T, Akhtar S, Nisar S, Sageena G, Ali S . Targeting cancer signaling pathways by natural products: Exploring promising anti-cancer agents. Biomed Pharmacother. 2022; 150:113054. DOI: 10.1016/j.biopha.2022.113054. View

Siegel R, Miller K, Jemal A . Cancer statistics, 2020. CA Cancer J Clin. 2020; 70(1):7-30. DOI: 10.3322/caac.21590. View

Tong X, Tang R, Xiao M, Xu J, Wang W, Zhang B . Targeting cell death pathways for cancer therapy: recent developments in necroptosis, pyroptosis, ferroptosis, and cuproptosis research. J Hematol Oncol. 2022; 15(1):174. PMC: 9733270. DOI: 10.1186/s13045-022-01392-3. View

Halawani E . Potential effects of Saudi Shaoka ( honey against multi-drug-resistant bacteria and cancer cells in comparison to Manuka honey. Saudi J Biol Sci. 2021; 28(12):7379-7389. PMC: 8626341. DOI: 10.1016/j.sjbs.2021.08.055. View

Tashkandi H . Honey in wound healing: An updated review. Open Life Sci. 2021; 16(1):1091-1100. PMC: 8496555. DOI: 10.1515/biol-2021-0084. View

Rio D, Ares Jr M, Hannon G, Nilsen T . Purification of RNA using TRIzol (TRI reagent). Cold Spring Harb Protoc. 2010; 2010(6):pdb.prot5439. DOI: 10.1101/pdb.prot5439. View

Martinotti S, Pellavio G, Patrone M, Laforenza U, Ranzato E . Manuka Honey Induces Apoptosis of Epithelial Cancer Cells through Aquaporin-3 and Calcium Signaling. Life (Basel). 2020; 10(11). PMC: 7692226. DOI: 10.3390/life10110256. View

10.

Almasaudi S . The antibacterial activities of honey. Saudi J Biol Sci. 2021; 28(4):2188-2196. PMC: 8071826. DOI: 10.1016/j.sjbs.2020.10.017. View

11.

Nasr F, Shahat A, Alqahtani A, Ahmed M, Qamar W, Al-Mishari A . Centaurea bruguierana inhibits cell proliferation, causes cell cycle arrest, and induces apoptosis in human MCF-7 breast carcinoma cells. Mol Biol Rep. 2020; 47(8):6043-6051. DOI: 10.1007/s11033-020-05679-x. View

12.

Canga I, Vita P, Oliveira A, Castro M, Pinho C . In Vitro Cytotoxic Activity of African Plants: A Review. Molecules. 2022; 27(15). PMC: 9370645. DOI: 10.3390/molecules27154989. View

13.

Zhong L, Li Y, Xiong L, Wang W, Wu M, Yuan T . Small molecules in targeted cancer therapy: advances, challenges, and future perspectives. Signal Transduct Target Ther. 2021; 6(1):201. PMC: 8165101. DOI: 10.1038/s41392-021-00572-w. View

14.

Taher M, Susanti D, Haris M, Rushdan A, Widodo R, Syukri Y . PEGylated liposomes enhance the effect of cytotoxic drug: A review. Heliyon. 2023; 9(3):e13823. PMC: 9976326. DOI: 10.1016/j.heliyon.2023.e13823. View

15.

Aryappalli P, Shabbiri K, Masad R, Al-Marri R, Haneefa S, Mohamed Y . Inhibition of Tyrosine-Phosphorylated STAT3 in Human Breast and Lung Cancer Cells by Manuka Honey is Mediated by Selective Antagonism of the IL-6 Receptor. Int J Mol Sci. 2019; 20(18). PMC: 6769459. DOI: 10.3390/ijms20184340. View

16.

Qian S, Wei Z, Yang W, Huang J, Yang Y, Wang J . The role of BCL-2 family proteins in regulating apoptosis and cancer therapy. Front Oncol. 2022; 12:985363. PMC: 9597512. DOI: 10.3389/fonc.2022.985363. View

17.

Mathew C, Tesfaye W, Rasmussen P, Peterson G, Bartholomaeus A, Sharma M . Mānuka Oil-A Review of Antimicrobial and Other Medicinal Properties. Pharmaceuticals (Basel). 2020; 13(11). PMC: 7694078. DOI: 10.3390/ph13110343. View

18.

Tomko A, Whynot E, Ellis L, Dupre D . Anti-Cancer Potential of Cannabinoids, Terpenes, and Flavonoids Present in Cannabis. Cancers (Basel). 2020; 12(7). PMC: 7409346. DOI: 10.3390/cancers12071985. View

19.

Al-Abkal F, Abdel-Wahab B, Abd El-Kareem H, Moustafa Y, Khodeer D . Protective Effect of Pycnogenol against Methotrexate-Induced Hepatic, Renal, and Cardiac Toxicity: An In Vivo Study. Pharmaceuticals (Basel). 2022; 15(6). PMC: 9229807. DOI: 10.3390/ph15060674. View

20.

Perini G, Ribeiro G, Pinto Neto J, Campos L, Hamerschlak N . BCL-2 as therapeutic target for hematological malignancies. J Hematol Oncol. 2018; 11(1):65. PMC: 5946445. DOI: 10.1186/s13045-018-0608-2. View