Genetic Diversity and Population Structure of : An Global Analysis

Overview

Journal J Adv Vet Anim Res

Specialty Veterinary Medicine

Date 2024 Aug 5

PMID 39101071

Authors

Ayed Alshammari

Muhammad Irshad Subhani

Majed H Wakid

Abdulsalam A M Alkhaldi

Shujaat Hussain

Muhammad Abdullah Malik

Muhammad Saqib

Warda Qamar

Mughees Aizaz Alvi

Affiliations

Soon will be listed here.

Abstract

Objective: Alveolar echinococcosis is caused by , a parasite of zoonotic significance with a wide range of intermediate and final hosts, and the parasite survives successfully in diversified conditions. Plentiful studies have been done to study the genetic structure of the population of the parasite and the level of intimate kinship using mitochondrial (mt) DNA. The present study was conducted to investigate the population structure, genetic variation, and phylogenetic relationship of various isolates of submitted to GenBank worldwide. Sequences of mt genes (mt-cytochrome c oxidase (1), mt-NADH dehydrogenase 1)) of were analyzed to achieve the set goals.

Materials And Methods: A total of 275 and 124 gene sequences of mt-1 and mt-1 belonging to . respectively, were retrieved from the National Center for Biotechnology Information GenBank. The retrieved sequences were subjected to alignment with respective reference sequences using MEGA software. The PopArt software was used to establish median-joining networks, while DnaSp was used to calculate neutrality and diversity indices. MrBayes software was used to investigate the phylogenetic association between haplotypes based on Bayesian phylogeny.

Results: Approximately 13 and 20 distinctive haplotypes of 1 and 1 genes, respectively, were observed in the present study. In both of the mt genes, diversity indices indicated low haplotype (mt-1 = 0.140; mt-1 = 0.374) and nucleotide (mt-1 = 0.00111; mt-1 = 0.00287) diversities. The values of Tajima's D and Fu Fs for a population of both of the genes under study were found to be negative.

Conclusion: This study is a maiden attempt to provide insights into the population structure and genetic variation of on a global scale. However, it is suggested that to better understand the population structure and genetic diversity of . more geographical locations and amplifications of full-length gene sequences should be considered, which could be helpful in widening the insights into the genetic diversity of . .

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References

Rozas J, Ferrer-Mata A, Sanchez-DelBarrio J, Guirao-Rico S, Librado P, Ramos-Onsins S . DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets. Mol Biol Evol. 2017; 34(12):3299-3302. DOI: 10.1093/molbev/msx248. View

Fu Y . Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics. 1997; 147(2):915-25. PMC: 1208208. DOI: 10.1093/genetics/147.2.915. View

Baumann S, Shi R, Liu W, Bao H, Schmidberger J, Kratzer W . Worldwide literature on epidemiology of human alveolar echinococcosis: a systematic review of research published in the twenty-first century. Infection. 2019; 47(5):703-727. PMC: 8505309. DOI: 10.1007/s15010-019-01325-2. View

Kinkar L, Laurimae T, Acosta-Jamett G, Andresiuk V, Balkaya I, Casulli A . Global phylogeography and genetic diversity of the zoonotic tapeworm Echinococcus granulosus sensu stricto genotype G1. Int J Parasitol. 2018; 48(9-10):729-742. DOI: 10.1016/j.ijpara.2018.03.006. View

Huelsenbeck J, Ronquist F . MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001; 17(8):754-5. DOI: 10.1093/bioinformatics/17.8.754. View

Romig T, Ebi D, Wassermann M . Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Vet Parasitol. 2015; 213(3-4):76-84. DOI: 10.1016/j.vetpar.2015.07.035. View

Vamathevan J, Hasan S, Emes R, Amrine-Madsen H, Rajagopalan D, Topp S . The role of positive selection in determining the molecular cause of species differences in disease. BMC Evol Biol. 2008; 8:273. PMC: 2576240. DOI: 10.1186/1471-2148-8-273. View

Jia W, Yan H, Lou Z, Ni X, Dyachenko V, Li H . Mitochondrial genes and genomes support a cryptic species of tapeworm within Taenia taeniaeformis. Acta Trop. 2012; 123(3):154-63. DOI: 10.1016/j.actatropica.2012.04.006. View

Ramos-Onsins S, Rozas J . Statistical properties of new neutrality tests against population growth. Mol Biol Evol. 2002; 19(12):2092-100. DOI: 10.1093/oxfordjournals.molbev.a004034. View

10.

Lymbery A . Phylogenetic Pattern, Evolutionary Processes and Species Delimitation in the Genus Echinococcus. Adv Parasitol. 2017; 95:111-145. DOI: 10.1016/bs.apar.2016.07.002. View

11.

Deplazes P, Hegglin D, Gloor S, Romig T . Wilderness in the city: the urbanization of Echinococcus multilocularis. Trends Parasitol. 2004; 20(2):77-84. DOI: 10.1016/j.pt.2003.11.011. View

12.

Vuitton D, McManus D, Rogan M, Romig T, Gottstein B, Naidich A . International consensus on terminology to be used in the field of echinococcoses. Parasite. 2020; 27:41. PMC: 7273836. DOI: 10.1051/parasite/2020024. View

13.

Luong L, Chambers J, Moizis A, Stock T, St Clair C . Helminth parasites and zoonotic risk associated with urban coyotes () in Alberta, Canada. J Helminthol. 2018; 94:e25. DOI: 10.1017/S0022149X1800113X. View

14.

Torgerson P, Keller K, Magnotta M, Ragland N . The global burden of alveolar echinococcosis. PLoS Negl Trop Dis. 2010; 4(6):e722. PMC: 2889826. DOI: 10.1371/journal.pntd.0000722. View

15.

Yimam A, Nonaka N, Oku Y, Kamiya M . Prevalence and intensity of Echinococcus multilocularis in red foxes (Vulpes vulpes schrencki) and raccoon dogs (Nyctereutes procyonoides albus) in Otaru City, Hokkaido, Japan. Jpn J Vet Res. 2002; 49(4):287-96. View

16.

Spotin A, Karamat M, Mahami-Oskouei M, Shahbazi A, Ahmadpour E, Galeh T . Genetic variability and transcontinental sharing of Giardia duodenalis infrapopulations determined by glutamate dehydrogenase gene. Acta Trop. 2017; 177:146-156. DOI: 10.1016/j.actatropica.2017.10.001. View

17.

Davidson R, Romig T, Jenkins E, Tryland M, Robertson L . The impact of globalisation on the distribution of Echinococcus multilocularis. Trends Parasitol. 2012; 28(6):239-47. DOI: 10.1016/j.pt.2012.03.004. View

18.

Yanagida T, Mohammadzadeh T, Kamhawi S, Nakao M, Sadjjadi S, Hijjawi N . Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitol Int. 2012; 61(4):599-603. DOI: 10.1016/j.parint.2012.05.014. View

19.

Andresiuk M, Gordo F, Saarma M, Elissondo M, Taraborelli A, Casalongue C . Echinococcus granulosus genotype G1 dominated in cattle and sheep during 2003-2006 in Buenos Aires province, an endemic area for cystic echinococcosis in Argentina. Acta Trop. 2013; 127(2):136-42. DOI: 10.1016/j.actatropica.2013.04.008. View

20.

Alvi M, Ohiolei J, Saqib M, Li L, Tayyab M, Alvi A . Echinococcus granulosus (sensu stricto) (G1, G3) and E. ortleppi (G5) in Pakistan: phylogeny, genetic diversity and population structural analysis based on mitochondrial DNA. Parasit Vectors. 2020; 13(1):347. PMC: 7359271. DOI: 10.1186/s13071-020-04199-8. View