Exophiala Species in Household Environments and Their Antifungal Resistance Profile

Overview

Journal Sci Rep

Specialty Science

Date 2024 Jul 31

PMID 39085337

Authors

Nahid Kondori

Daniel Jaen-Luchoro

Roger Karlsson

Bahman Abedzaedeh

Helena Hammarstrom

Bodil Jonsson

Affiliations

Soon will be listed here.

Abstract

The black fungus Exophiala causes a wide range of infections from superficial to subcutaneous, but also invasive fungal infections in immunocompromised patients as well as healthy individuals. In addition, Exophiala, is a common colonizer of the air ways of patients with cystic fibrosis. However, the source of infection and mode of transmission is still unclear. The aim of this study was to investigate the presence of Exophiala in samples collected from Swedish indoor environments. We found that the Exophiala species were commonly found in dishwashers and that Exophiala dermatitidis was the most common Exophiala species, being identified in 70% (26 out of the 37) of samples. Almost all E. dermatitidis isolates had the ability to grow at 42 °C (P = 0.0002) and were catalase positive. Voriconazole and posaconazole exhibited the lowest MICs, while caspofungin and anidulafungin lack the antifungal activities in vitro. Future studies are needed to illuminate the transmission mode of the fungi.

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References

Kondori N, Gilljam M, Lindblad A, Jonsson B, Moore E, Wenneras C . High rate of Exophiala dermatitidis recovery in the airways of patients with cystic fibrosis is associated with pancreatic insufficiency. J Clin Microbiol. 2011; 49(3):1004-9. PMC: 3067733. DOI: 10.1128/JCM.01899-10. View

Usuda D, Higashikawa T, Hotchi Y, Usami K, Shimozawa S, Tokunaga S . Exophiala dermatitidis. World J Clin Cases. 2021; 9(27):7963-7972. PMC: 8462220. DOI: 10.12998/wjcc.v9.i27.7963. View

Novak Babic M, Zupancic J, Gunde-Cimerman N, de Hoog S, Zalar P . Ecology of the Human Opportunistic Black Yeast Exophiala dermatitidis Indicates Preference for Human-Made Habitats. Mycopathologia. 2017; 183(1):201-212. DOI: 10.1007/s11046-017-0134-8. View

Hamilton A, Holdom M . Antioxidant systems in the pathogenic fungi of man and their role in virulence. Med Mycol. 2000; 37(6):375-89. DOI: 10.1046/j.1365-280x.1999.00208.x. View

Jeffries C, HOLTMAN D, GUSE D . Rapid method for determining the activity of microorganisms on nucleic acids. J Bacteriol. 1957; 73(4):590-1. PMC: 314626. DOI: 10.1128/jb.73.4.590-591.1957. View

Casal M, Linares M . Preliminary evaluation of a new test for the rapid differentiation of Candida albicans and Candida stellatoidea. Mycopathologia. 1983; 81(1):63-4. DOI: 10.1007/BF00443910. View

Kondori N, Lindblad A, Welinder-Olsson C, Wenneras C, Gilljam M . Development of IgG antibodies to Exophiala dermatitidis is associated with inflammatory responses in patients with cystic fibrosis. J Cyst Fibros. 2014; 13(4):391-9. DOI: 10.1016/j.jcf.2013.12.007. View

Zalar P, Novak M, de Hoog G, Gunde-Cimerman N . Dishwashers--a man-made ecological niche accommodating human opportunistic fungal pathogens. Fungal Biol. 2011; 115(10):997-1007. DOI: 10.1016/j.funbio.2011.04.007. View

Matos T, de Hoog G, De Boer A, de Crom I, Haase G . High prevalence of the neurotrope Exophiala dermatitidis and related oligotrophic black yeasts in sauna facilities. Mycoses. 2002; 45(9-10):373-7. DOI: 10.1046/j.1439-0507.2002.00779.x. View

10.

Tesei D, Marzban G, Marchetti-Deschmann M, Tafer H, Arcalis E, Sterflinger K . Proteome of tolerance fine-tuning in the human pathogen black yeast Exophiala dermatitidis. J Proteomics. 2015; 128:39-57. DOI: 10.1016/j.jprot.2015.07.007. View

11.

Caston-Osorio J, Rivero A, Torre-Cisneros J . Epidemiology of invasive fungal infection. Int J Antimicrob Agents. 2008; 32 Suppl 2:S103-9. DOI: 10.1016/S0924-8579(08)70009-8. View

12.

Schwarz C, Bouchara J, Buzina W, Chrenkova V, Dmenska H, de la Pedrosa E . Organization of Patient Management and Fungal Epidemiology in Cystic Fibrosis. Mycopathologia. 2017; 183(1):7-19. PMC: 7089279. DOI: 10.1007/s11046-017-0205-x. View

13.

Hashimoto K, Fujii H, Kawakami Y . Genetic Identification of Dematiaceous Fungi Isolated from Washing Machine in Japan, and Considering of Fungal Removal Methods. Biocontrol Sci. 2019; 24(2):89-96. DOI: 10.4265/bio.24.89. View

14.

Dogen A, Kaplan E, Oksuz Z, Serin M, Ilkit M, de Hoog G . Dishwashers are a major source of human opportunistic yeast-like fungi in indoor environments in Mersin, Turkey. Med Mycol. 2012; 51(5):493-8. DOI: 10.3109/13693786.2012.738313. View

15.

. Exophiala infection from contaminated injectable steroids prepared by a compounding pharmacy--United States, July-November 2002. MMWR Morb Mortal Wkly Rep. 2003; 51(49):1109-12. View

16.

Sav H, Ozakkas F, Altinbas R, Kiraz N, Tumgor A, Gumral R . Virulence markers of opportunistic black yeast in Exophiala. Mycoses. 2016; 59(6):343-50. DOI: 10.1111/myc.12478. View

17.

Sudhadham M, Prakitsin S, Sivichai S, Chaiyarat R, Dorrestein G, Menken S . The neurotropic black yeast Exophiala dermatitidis has a possible origin in the tropical rain forest. Stud Mycol. 2009; 61:145-55. PMC: 2610309. DOI: 10.3114/sim.2008.61.15. View

18.

Badali H, de Hoog G, Sudhadham M, Meis J . Microdilution in vitro antifungal susceptibility of Exophiala dermatitidis, a systemic opportunist. Med Mycol. 2011; 49(8):819-24. DOI: 10.3109/13693786.2011.583285. View

19.

Bongomin F, Gago S, Oladele R, Denning D . Global and Multi-National Prevalence of Fungal Diseases-Estimate Precision. J Fungi (Basel). 2018; 3(4). PMC: 5753159. DOI: 10.3390/jof3040057. View