Investigation of Symmetric Dimethylarginine As a Serologic Marker for Kidney Function in Striped Skunks ()

Overview

Journal J Vet Sci

Specialty Veterinary Medicine

Date 2024 Jul 31

PMID 39083204

Authors

Eun Jung

Soong-Hee Youn

Ki-Yong Shin

Hyeon-Joo Shin

Joon-Young Yang

Yeseul Yang

Jae-Ha Jung

Yongbaek Kim

Affiliations

Soon will be listed here.

Abstract

Importance: Kidney disease is prevalent among veterinary species, including zoo animals; however, investigations into this condition in striped skunks () are scarce. Diagnostic tools for kidney diseases in this species also remain limited.

Objective: This study aimed to assess the utility of symmetric dimethylarginine as a biomarker for kidney disease in captive striped skunks in Korea.

Methods: This retrospective study analysed 11 striped skunks housed at the Everland Zoo between 2017 and 2021. Blood samples were collected during health checks. Kidney function was assessed through blood analysis and diagnostic ultrasound, with necropsies conducted on deceased animals. Symmetric dimethylarginine levels were measured in 27 plasma samples collected from 11 skunks.

Results: Over the study period, seven skunks were diagnosed with kidney disease. Analysis of 27 blood samples revealed a concurrent increase in SDMA levels with concentrations of blood urea nitrogen and blood creatinine. In 3 of the 7 skunks with kidney disease, symmetric dimethylarginine exceeded 14 µg/dL prior to the elevation of blood urea nitrogen and blood creatinine above the upper reference limit.

Conclusions And Relevance: To our knowledge, this is the first study investigating symmetric dimethylarginine in captive striped skunks in Korea. Our findings suggest that symmetric dimethylarginine may serve as an early and consistent biomarker for renal dysfunction in striped skunks. Further studies with larger clinical sample size from striped skunks are needed to validate the clinical utility of blood symmetric dimethylarginine concentration.

References

Braff J, Obare E, Elliott J, Yerramilli M . Relationship between serum symmetric dimethylarginine concentration and glomerular filtration rate in cats. J Vet Intern Med. 2014; 28(6):1699-701. PMC: 4895615. DOI: 10.1111/jvim.12446. View

Hall J, Obare E, Yerramilli M, Jewell D . Comparison of serum concentrations of symmetric dimethylarginine and creatinine as kidney function biomarkers in cats with chronic kidney disease. J Vet Intern Med. 2014; 28(6):1676-83. PMC: 4895610. DOI: 10.1111/jvim.12445. View

El-Khoury J, Bunch D, Reineks E, Jackson R, Steinle R, Wang S . A simple and fast liquid chromatography-tandem mass spectrometry method for measurement of underivatized L-arginine, symmetric dimethylarginine, and asymmetric dimethylarginine and establishment of the reference ranges. Anal Bioanal Chem. 2011; 402(2):771-9. DOI: 10.1007/s00216-011-5462-9. View

Hokamp J, Nabity M . Renal biomarkers in domestic species. Vet Clin Pathol. 2016; 45(1):28-56. DOI: 10.1111/vcp.12333. View

Lamglait B, Vandenbunder-Beltrame M . EVALUATION OF SYMMETRIC DIMETHYLARGININE AS AN EARLY BIOMARKER OF CHRONIC KIDNEY DISEASE IN CAPTIVE CHEETAHS (ACINONYX JUBATUS). J Zoo Wildl Med. 2017; 48(3):874-877. DOI: 10.1638/2016-0224.1. View

ONeill D, Elliott J, Church D, McGreevy P, Thomson P, Brodbelt D . Chronic kidney disease in dogs in UK veterinary practices: prevalence, risk factors, and survival. J Vet Intern Med. 2013; 27(4):814-21. DOI: 10.1111/jvim.12090. View

Brown C, Elliott J, Schmiedt C, Brown S . Chronic Kidney Disease in Aged Cats: Clinical Features, Morphology, and Proposed Pathogeneses. Vet Pathol. 2016; 53(2):309-26. DOI: 10.1177/0300985815622975. View

Hall J, Obare E, Yerramilli M, Almes K, Jewell D . Serum Concentrations of Symmetric Dimethylarginine and Creatinine in Dogs with Naturally Occurring Chronic Kidney Disease. J Vet Intern Med. 2016; 30(3):794-802. PMC: 4913574. DOI: 10.1111/jvim.13942. View

Pouchelon J, Atkins C, Bussadori C, Oyama M, Vaden S, Bonagura J . Cardiovascular-renal axis disorders in the domestic dog and cat: a veterinary consensus statement. J Small Anim Pract. 2015; 56(9):537-52. PMC: 4584495. DOI: 10.1111/jsap.12387. View

10.

Kakimoto Y, Akazawa S . Isolation and identification of N-G,N-G- and N-G,N'-G-dimethyl-arginine, N-epsilon-mono-, di-, and trimethyllysine, and glucosylgalactosyl- and galactosyl-delta-hydroxylysine from human urine. J Biol Chem. 1970; 245(21):5751-8. View

11.

Schott 2nd H, Gallant L, Coyne M, Murphy R, Cross J, Strong-Townsend M . Symmetric dimethylarginine and creatinine concentrations in serum of healthy draft horses. J Vet Intern Med. 2021; 35(2):1147-1154. PMC: 7995414. DOI: 10.1111/jvim.16042. View

12.

Nabity M, Lees G, Boggess M, Obare E, Yerramilli M, Rakitin A . Symmetric Dimethylarginine Assay Validation, Stability, and Evaluation as a Marker for the Early Detection of Chronic Kidney Disease in Dogs. J Vet Intern Med. 2015; 29(4):1036-44. PMC: 4895368. DOI: 10.1111/jvim.12835. View

13.

Crowell W, Stuart B, ADAMS W . Renal lesions in striped skunks (Mephitis mephitis) from Louisiana. J Wildl Dis. 1977; 13(3):300-3. DOI: 10.7589/0090-3558-13.3.300. View

14.

Mota S, Brandao J, Guthrie A . COMPARISON OF BLOOD SYMMETRIC DIMETHYLARGININE AND CREATININE AS ENDOGENOUS MARKERS OF KIDNEY FUNCTION IN CAPTIVE TIGERS (). J Zoo Wildl Med. 2021; 52(2):628-637. DOI: 10.1638/2020-0032. View

15.

van Zanten T, Xie S . EVALUATION OF SYMMETRIC DIMETHYLARGININE AS AN ENDOGENOUS MARKER OF RENAL DISEASE IN GREATER FLAMINGOS (). J Zoo Wildl Med. 2023; 53(4):644-653. DOI: 10.1638/2021-0134. View

16.

Choi B, Moon H, Seo S, Hyun C . Evaluation of serum cystatin-C and symmetric dimethylarginine concentrations in dogs with heart failure from chronic mitral valvular insufficiency. J Vet Med Sci. 2016; 79(1):41-46. PMC: 5289235. DOI: 10.1292/jvms.16-0188. View

17.

Dahlem D, Neiger R, Schweighauser A, Francey T, Yerramilli M, Obare E . Plasma Symmetric Dimethylarginine Concentration in Dogs with Acute Kidney Injury and Chronic Kidney Disease. J Vet Intern Med. 2017; 31(3):799-804. PMC: 5435041. DOI: 10.1111/jvim.14694. View

18.

Kaiser F, de le Roi M, Jo W, Gerhauser I, Molnar V, Osterhaus A . First Report of Skunk Amdoparvovirus (Species ) in Europe in a Captive Striped Skunk (). Viruses. 2023; 15(5). PMC: 10221443. DOI: 10.3390/v15051087. View

19.

Hall J, Yerramilli M, Obare E, Yerramilli M, Melendez L, Jewell D . Relationship between lean body mass and serum renal biomarkers in healthy dogs. J Vet Intern Med. 2015; 29(3):808-14. PMC: 4895404. DOI: 10.1111/jvim.12607. View

20.

Pennick K, Latimer K, Brown C, Hayes J, Sarver C . Aleutian disease in two domestic striped skunks (Mephitis mephitis). Vet Pathol. 2007; 44(5):687-90. DOI: 10.1354/vp.44-5-687. View