Green Synthesized Extracts/Au Complex of : Unrevealing the Anti-cancer and Anti-bacterial Potentialities, Supported by Metabolomics and Molecular Modeling

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Jul 29

PMID 39071630

Authors

Sultan Aati

Hanan Y Aati

Sherine El-Shamy

Mohammad A Khanfar

Mohamed A Ghani A Naeim

Ahmed A Hamed

Mostafa E Rateb

Hossam M Hassan

Mahmoud A Aboseada

Affiliations

Soon will be listed here.

Abstract

The anti-cancer and anti-bacterial potential of the Red Sea sponge in its bulk (crude extracts) and gold nanostructure (loaded on gold nanaoparticles) were investigated. Metabolomics analysis was conducted, and subsequently, molecular modeling studies were conducted to explore and anticipate the secondary metabolites and their potential target for their various bioactivities. The chloroformic extract (CE) and ethyl acetate extract (EE) of the predicted to include bioactive lipophilic and moderately polar metabolites, respectively, were used to synthesize gold nanoparticles (AuNPs). The prepared AuNPs were characterized through transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and UV-vis spectrophotometric analyses. The cytotoxic activities were tested against MCF-7, MDB-231, and MCF-10A. Moreover, the anti-bacterial, antifungal, and anti-biofilm activity were assessed. Definite classes of metabolites were identified in CE (terpenoids) and EE (brominated phenyl ethers and sulfated fatty amides). Molecular modeling involving docking and molecular dynamics identified Protein-tyrosine phosphatase 1B (PTP1B) as a potential target for the anti-cancer activities of terpenoids. Moreover, CE exhibited the most powerful activity against breast cancer cell lines, matching our molecular modeling study. On the other hand, only EE was demonstrated to possess powerful anti-bacterial and anti-biofilm activity against . In conclusion, depending on their bioactive metabolites, -derived extracts, after being loaded on AuNPs, could be considered anti-cancer, anti-bacterial, and anti-biofilm bioactive products. Future work should be completed to produce drug leads.

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