Green Synthesis of Chitosan/Silver Nanoparticles Using Extract and Its Potential Anti-Cryptosporidiosis Effect

Overview

Journal Pharmaceutics

Publisher MDPI

Date 2024 Jul 27

PMID 39065665

Authors

Muslimah N Alsulami

Eman S El-Wakil

Affiliations

Soon will be listed here.

Abstract

() is one of the most prevalent species infecting humans and animals. Currently, the only FDA-licensed drug to treat cryptosporidiosis is nitazoxanide (NTZ), with no efficacy in immunocompromised hosts. () has demonstrated anti-protozoal activities. This study aimed to investigate the anti-cryptosporidiosis effect of peel extract, either alone or in mediating the green synthesis of chitosan silver nanoparticles (Cs/Ag NPs), compared to NTZ. Mice were sorted into nine different groups. The effectiveness of the treatments was evaluated using parasitology, histopathology, immunohistochemistry, and immunology. outperformed nitazoxanide regarding oocyst shedding (79% vs. 61%). The effectiveness of NTZ Cs/Ag NPs and Citrus Cs/Ag NPs was enhanced to 78% and 91%, respectively. The highest oocyst inhibition was obtained by combining NTZ and Citrus Cs/Ag NPs (96%). NF-κB, TNF-α, and Il-10 levels increased in response to infection and decreased in response to various treatments, with the highest reduction in the group treated with combined NTZ citrus Cs/Ag NPs. Combining with NTZ could have a synergistic effect, making it a potentially effective anti-cryptosporidiosis agent. Utilizing in the green synthesis of Cs/Ag NPs improves the therapeutic response and can be used to produce novel therapeutic antiparasitic drugs.

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