The Role of Gene Family in Abiotic Stress Response

The DOF (DNA-binding with one finger) transcription factors are exclusive to plants and play crucial roles in plant growth, development, and environmental adaptation. Although extensive research has been conducted on the gene family in , , and , investigations concerning the role of this gene family in remain unreported, leaving its biological function largely unknown. In this study, we performed a comprehensive genome-wide identification of the gene family based on the genome, resulting in the discovery of a total of 17 gene members. Based on the results of phylogenetic analysis, the 17 LcDof proteins were classified into eight subfamilies. The motif analysis revealed the diverse nature of motifs within the D1 subfamily, which includes a distinct type of Dof transcription factor known as (Cycling Dof Factor). We further characterized the chromosomal distribution, gene structure, conserved protein motifs, and cis-elements in the promoter regions. Additionally, utilizing transcriptome data from hybrids and conducting RT-qPCR experiments, we investigated the expression patterns of under various abiotic stresses such as drought, cold, and heat stress. Notably, we found that several , particularly and , were significantly upregulated in response to abiotic stress. Furthermore, we cloned and genes and found that its encoding protein was mainly located in the nucleus by transient transformation in hybrids protoplast. Subsequently, we used -overexpressing hybrid seedlings. We found that overexpression of enhanced the cold tolerance of the plants, increasing their survival rate at -20 °C. This result was further validated by changes in physiological indicators.