Effects of Phloretin on Seedling Growth and Histochemical Distribution of Phenols, Polysaccharides and Lipids in (L.) Medik

Overview

Journal Plants (Basel)

Date 2024 Jul 27

PMID 39065417

Authors

Milica dordic

Dusica Janosevic

Dijana Smailagic

Nevena Banjac

Slavica Ninkovic

Mariana Stanisic

Milena Trajkovic

Affiliations

Soon will be listed here.

Abstract

The present study evaluates the phytotoxic effects of phloretin, a prevalent secondary metabolite of apple trees, on the broadleaf weed (L.) Medik. known for its resistant myxospermous seeds that form a long-lasting soil bank. The results indicate a significant, dose-dependent inhibitory effect of phloretin on the growth and morphological parameters of weed seedlings grown in vitro. Although the applied phloretin concentrations (250-1000 µM) were not lethal to the seedlings after two weeks, the metabolism of the seedlings was impaired, resulting in an accumulation of lipid droplets in the root tips and root hairs. Histochemical analysis shows deposits of phenols in the root epidermal cells, which are probably aggregates of phloretin or its metabolic derivatives. The accumulation of pectin in the cell walls of root border cells in phloretin-treated seedlings indicates an attempt to reduce the uptake of phloretin and reduce its concentration in the cells. Inhibition of shoot growth associated with chlorosis and reduced photosynthetic pigment content is a consequence of seedling exposure to phloretin. This study provides a basis for further evaluation of phloretin as a new bioherbicidal compound and for elucidating the mechanism underlying its phytotoxic activity.

References

Cheng F, Cheng Z . Research Progress on the use of Plant Allelopathy in Agriculture and the Physiological and Ecological Mechanisms of Allelopathy. Front Plant Sci. 2015; 6:1020. PMC: 4647110. DOI: 10.3389/fpls.2015.01020. View

Wang Q, Xu Z, Hu T, Ur Rehman H, Chen H, Li Z . Allelopathic activity and chemical constituents of walnut (Juglans regia) leaf litter in walnut-winter vegetable agroforestry system. Nat Prod Res. 2014; 28(22):2017-20. DOI: 10.1080/14786419.2014.913245. View

Fry S . Feruloylated pectins from the primary cell wall: their structure and possible functions. Planta. 2013; 157(2):111-23. DOI: 10.1007/BF00393644. View

Yang X, Dong M, Huang Z . Role of mucilage in the germination of Artemisia sphaerocephala (Asteraceae) achenes exposed to osmotic stress and salinity. Plant Physiol Biochem. 2010; 48(2-3):131-5. DOI: 10.1016/j.plaphy.2009.12.006. View

Fernandes P, Le Bourvellec C, Renard C, Wessel D, Cardoso S, Coimbra M . Interactions of arabinan-rich pectic polysaccharides with polyphenols. Carbohydr Polym. 2020; 230:115644. DOI: 10.1016/j.carbpol.2019.115644. View

Yin C, Xiang L, Wang G, Wang Y, Shen X, Chen X . How to Plant Apple Trees to Reduce Replant Disease in Apple Orchard: A Study on the Phenolic Acid of the Replanted Apple Orchard. PLoS One. 2016; 11(12):e0167347. PMC: 5132267. DOI: 10.1371/journal.pone.0167347. View

Wesse C, Welk E, Hurka H, Neuffer B . Geographical pattern of genetic diversity in (Brassicaceae)-A global perspective. Ecol Evol. 2021; 11(1):199-213. PMC: 7790636. DOI: 10.1002/ece3.7010. View

Chang W, Huang W, Liou C . Evaluation of the anti-inflammatory effects of phloretin and phlorizin in lipopolysaccharide-stimulated mouse macrophages. Food Chem. 2012; 134(2):972-9. DOI: 10.1016/j.foodchem.2012.03.002. View

Ishii T . Isolation and characterization of a diferuloyl arabinoxylan hexasaccharide from bamboo shoot cell-walls. Carbohydr Res. 1991; 219:15-22. DOI: 10.1016/0008-6215(91)89039-i. View

10.

de Oliveira M . Phloretin-induced cytoprotective effects on mammalian cells: A mechanistic view and future directions. Biofactors. 2016; 42(1):13-40. DOI: 10.1002/biof.1256. View

11.

Sampath C, Rashid M, Sang S, Ahmedna M . Specific bioactive compounds in ginger and apple alleviate hyperglycemia in mice with high fat diet-induced obesity via Nrf2 mediated pathway. Food Chem. 2017; 226:79-88. DOI: 10.1016/j.foodchem.2017.01.056. View

12.

Boccia M, Kopf S, Baratti C . Phlorizin, a competitive inhibitor of glucose transport, facilitates memory storage in mice. Neurobiol Learn Mem. 1999; 71(1):104-12. DOI: 10.1006/nlme.1998.3856. View

13.

Puel C, Quintin A, MATHEY J, Obled C, Davicco M, Lebecque P . Prevention of bone loss by phloridzin, an apple polyphenol, in ovariectomized rats under inflammation conditions. Calcif Tissue Int. 2005; 77(5):311-8. DOI: 10.1007/s00223-005-0060-5. View

14.

Windsor J, Symonds V, Mendenhall J, Lloyd A . Arabidopsis seed coat development: morphological differentiation of the outer integument. Plant J. 2000; 22(6):483-93. DOI: 10.1046/j.1365-313x.2000.00756.x. View

15.

Jones P, Vogt T . Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers. Planta. 2001; 213(2):164-74. DOI: 10.1007/s004250000492. View

16.

Na X, Hu Y, Yue K, Lu H, Jia P, Wang H . Narciclasine modulates polar auxin transport in Arabidopsis roots. J Plant Physiol. 2011; 168(11):1149-56. DOI: 10.1016/j.jplph.2011.01.025. View

17.

Gaucher M, Duge de Bernonville T, Lohou D, Guyot S, Guillemette T, Brisset M . Histolocalization and physico-chemical characterization of dihydrochalcones: Insight into the role of apple major flavonoids. Phytochemistry. 2013; 90:78-89. DOI: 10.1016/j.phytochem.2013.02.009. View

18.

Grana E, Sotelo T, Diaz-Tielas C, Araniti F, Krasuska U, Bogatek R . Citral induces auxin and ethylene-mediated malformations and arrests cell division in Arabidopsis thaliana roots. J Chem Ecol. 2013; 39(2):271-82. DOI: 10.1007/s10886-013-0250-y. View

19.

Rezk B, Haenen G, van der Vijgh W, Bast A . The antioxidant activity of phloretin: the disclosure of a new antioxidant pharmacophore in flavonoids. Biochem Biophys Res Commun. 2002; 295(1):9-13. DOI: 10.1016/s0006-291x(02)00618-6. View

20.

Renard C, Baron A, Guyot S, Drilleau J . Interactions between apple cell walls and native apple polyphenols: quantification and some consequences. Int J Biol Macromol. 2001; 29(2):115-25. DOI: 10.1016/s0141-8130(01)00155-6. View