Comparative Analysis of Vector of Leishmaniasis in India and Sri Lanka

Overview

Journal Microorganisms

Specialty Microbiology

Date 2024 Jul 27

PMID 39065228

Authors

Sachee Bhanu Piyasiri

P A Fathima

Harish Kumar Shah

Sanath Senanyake

Nilakshi Samaranayake

Nadira Darshani Karunaweera

Manju Rahi

Prasanta Saini

Affiliations

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Abstract

is the predominant sandfly vector of leishmaniasis in the Indian subcontinent. India and Sri Lanka primarily report visceral and cutaneous leishmaniasis caused by . We compared from two locations, focusing on its morphological, molecular, and salivary protein characteristics. Sandflies were captured using CDC light traps and cattle-baited net traps. Species identification and morphological comparisons were carried out using standard taxonomic keys. DNA extracted from 12 Sri Lankan sandfly samples was PCR-amplified and sequenced for the variable region of Cytochrome oxidase subunit I. Existing DNA sequences of India from GenBank were utilized for a phylogenetic analysis between Sri Lanka and India. Salivary protein profiles were studied using SDS-PAGE, Western blot, and electrospray ionization/LC/MS/MS. The morphological similarities observed between female from India and Sri Lanka suggest the presence of var. . A phylogenetic analysis showed genetic divergence between populations, but both shared a similar salivary protein profile. A common, strong 30 kDa immunogenic band comprised PagSP05, PagSP06, and PagSP17 proteins of . The similarity between the immunogenic salivary proteins suggests their potential use as common markers for vector exposure or immune response stimulants across regions. The use of multiple samples for each category of serum would improve the comprehensiveness of the immunogenic profiles obtained.

Citing Articles

Salivary antigens rPagSP02 and rPagSP06 are a reliable composite biomarker for evaluating exposure to Phlebotomus argentipes in Sri Lanka.

Piyasiri S, Senanayake S, Smaranayake N, Doh S, Iniguez E, Valenzuela J Sci Rep. 2024; 14(1):25863.

PMID: 39468289 PMC: 11519893. DOI: 10.1038/s41598-024-77666-2.

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