Hydrogen-Rich Gas Enhanced Sprint-Interval Performance: Metabolomic Insights into Underlying Mechanisms

Overview

Journal Nutrients

Date 2024 Jul 27

PMID 39064785

Authors

Gengxin Dong

Haiyan Liu

Yunji Chen

Dapeng Bao

Wentao Xu

Junhong Zhou

Affiliations

Soon will be listed here.

Abstract

(1) Background: The diversity of blood biomarkers used to assess the metabolic mechanisms of hydrogen limits a comprehensive understanding of its effects on improving exercise performance. This study evaluated the impact of hydrogen-rich gas (HRG) on metabolites following sprint-interval exercise using metabolomics approaches, aiming to elucidate its underlying mechanisms of action. (2) Methods: Ten healthy adult males participated in the Wingate Sprint-interval test (SIT) following 60 min of HRG or placebo (air) inhalation. Venous blood samples were collected for metabolomic analysis both before and after gas inhalation and subsequent to completing the SIT. (3) Results: Compared with the placebo, HRG inhalation significantly improved mean power, fatigue index, and time to peak for the fourth sprint and significantly reduced the attenuation values of peak power, mean power, and time to peak between the first and fourth. Metabolomic analysis highlighted the significant upregulation of acetylcarnitine, propionyl-L-carnitine, hypoxanthine, and xanthine upon HRG inhalation, with enrichment pathway analysis suggesting that HRG may foster fat mobilization by enhancing coenzyme A synthesis, promoting glycerophospholipid metabolism, and suppressing insulin levels. (4) Conclusions: Inhaling HRG before an SIT enhances end-stage anaerobic sprint capabilities and mitigates fatigue. Metabolomic analysis suggests that HRG may enhance ATP recovery during interval stages by accelerating fat oxidation, providing increased energy replenishment for late-stage sprints.

References

Sahlin K, Tonkonogi M, Soderlund K . Plasma hypoxanthine and ammonia in humans during prolonged exercise. Eur J Appl Physiol Occup Physiol. 1999; 80(5):417-22. DOI: 10.1007/s004210050613. View

Parker L, Trewin A, Levinger I, Shaw C, Stepto N . Exercise-intensity dependent alterations in plasma redox status do not reflect skeletal muscle redox-sensitive protein signaling. J Sci Med Sport. 2017; 21(4):416-421. DOI: 10.1016/j.jsams.2017.06.017. View

Allen J, Davey H, Broadhurst D, Heald J, Rowland J, Oliver S . High-throughput classification of yeast mutants for functional genomics using metabolic footprinting. Nat Biotechnol. 2003; 21(6):692-6. DOI: 10.1038/nbt823. View

Kamimura N, Nishimaki K, Ohsawa I, Ohta S . Molecular hydrogen improves obesity and diabetes by inducing hepatic FGF21 and stimulating energy metabolism in db/db mice. Obesity (Silver Spring). 2011; 19(7):1396-403. DOI: 10.1038/oby.2011.6. View

Bloomer R . Effect of exercise on oxidative stress biomarkers. Adv Clin Chem. 2008; 46:1-50. DOI: 10.1016/s0065-2423(08)00401-0. View

Zhou H, Zhang H, Ye R, Yan C, Lin J, Huang Y . Pantothenate protects against obesity via brown adipose tissue activation. Am J Physiol Endocrinol Metab. 2022; 323(1):E69-E79. DOI: 10.1152/ajpendo.00293.2021. View

Montes-de-Oca-Garcia A, Perez-Bey A, Corral-Perez J, Marin-Galindo A, Calderon-Dominguez M, Velazquez-Diaz D . Influence of Gender on Plasma Leptin Levels, Fat Oxidation, and Insulin Sensitivity in Young Adults: The Mediating Role of Fitness and Fatness. Nutrients. 2023; 15(11). PMC: 10255472. DOI: 10.3390/nu15112628. View

Kellmann M, Bertollo M, Bosquet L, Brink M, Coutts A, Duffield R . Recovery and Performance in Sport: Consensus Statement. Int J Sports Physiol Perform. 2018; 13(2):240-245. DOI: 10.1123/ijspp.2017-0759. View

Dong G, Wu J, Hong Y, Li Q, Liu M, Jiang G . Inhalation of Hydrogen-rich Gas before Acute Exercise Alleviates Exercise Fatigue: A Randomized Crossover Study. Int J Sports Med. 2024; 45(13):1014-1022. DOI: 10.1055/a-2318-1880. View

10.

Wang Q, Zha X, Kang Z, Xu M, Huang Q, Zou D . Therapeutic effects of hydrogen saturated saline on rat diabetic model and insulin resistant model via reduction of oxidative stress. Chin Med J (Engl). 2012; 125(9):1633-7. View

11.

Botek M, Khanna D, Krejci J, Valenta M, McKune A, Sladeckova B . Molecular Hydrogen Mitigates Performance Decrement during Repeated Sprints in Professional Soccer Players. Nutrients. 2022; 14(3). PMC: 8838970. DOI: 10.3390/nu14030508. View

12.

Fisher-Wellman K, Bloomer R . Acute exercise and oxidative stress: a 30 year history. Dyn Med. 2009; 8:1. PMC: 2642810. DOI: 10.1186/1476-5918-8-1. View

13.

Shibayama Y, Dobashi S, Arisawa T, Fukuoka T, Koyama K . Impact of hydrogen-rich gas mixture inhalation through nasal cannula during post-exercise recovery period on subsequent oxidative stress, muscle damage, and exercise performances in men. Med Gas Res. 2020; 10(4):155-162. PMC: 8092152. DOI: 10.4103/2045-9912.304222. View

14.

Wang L, Chen A, Li J, Sun Z, Yan S, Xu K . Mechanism of the Effect of High-Intensity Training on Urinary Metabolism in Female Water Polo Players Based on UHPLC-MS Non-Targeted Metabolomics Technique. Healthcare (Basel). 2021; 9(4). PMC: 8067095. DOI: 10.3390/healthcare9040381. View

15.

Dohi K, Kraemer B, Erickson M, McMillan P, Kovac A, Flachbartova Z . Molecular hydrogen in drinking water protects against neurodegenerative changes induced by traumatic brain injury. PLoS One. 2014; 9(9):e108034. PMC: 4176020. DOI: 10.1371/journal.pone.0108034. View

16.

Ohsawa I, Ishikawa M, Takahashi K, Watanabe M, Nishimaki K, Yamagata K . Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med. 2007; 13(6):688-94. DOI: 10.1038/nm1577. View

17.

Powers S, Deminice R, Ozdemir M, Yoshihara T, Bomkamp M, Hyatt H . Exercise-induced oxidative stress: Friend or foe?. J Sport Health Sci. 2020; 9(5):415-425. PMC: 7498668. DOI: 10.1016/j.jshs.2020.04.001. View

18.

Finsterer J . Biomarkers of peripheral muscle fatigue during exercise. BMC Musculoskelet Disord. 2012; 13:218. PMC: 3534479. DOI: 10.1186/1471-2474-13-218. View

19.

Aoki K, Nakao A, Adachi T, Matsui Y, Miyakawa S . Pilot study: Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes. Med Gas Res. 2012; 2:12. PMC: 3395574. DOI: 10.1186/2045-9912-2-12. View

20.

Hori A, Ichihara M, Kimura H, Ogata H, Kondo T, Hotta N . Inhalation of molecular hydrogen increases breath acetone excretion during submaximal exercise: a randomized, single-blinded, placebo-controlled study. Med Gas Res. 2020; 10(3):96-102. PMC: 8086628. DOI: 10.4103/2045-9912.296038. View