Monitoring of Immune Memory by Phenotypical Lymphocyte Subsets Identikit: An Observational Study in a Blood Donors' Cohort

Overview

Journal J Pers Med

Date 2024 Jul 27

PMID 39063987

Authors

Marina Di Domenico

Enrica Serretiello

Annafrancesca Smimmo

Fabio Franca Vieira E Silva

Sonia Anna Raimondi

Caterina Pascariello

Maria Michela Marino

Lorenzo Lo Muzio

Vito Carlo Alberto Caponio

Stefania Cantore

Andrea Ballini

Affiliations

Soon will be listed here.

Abstract

The cross-talk between the innate and adaptive immune response represents the first defense weapon against the threat of pathogens. Substantial evidence has shown a relationship between immune phenotype lymphocytes and COVID-19 disease severity and/or implication in susceptibility to SARS-CoV-2 infection. Recently, belonging to ABO blood groups has been investigated as a correlation factor to COVID-19 disease. This pilot study investigated lymphocyte typing in a cohort of blood donors to understand the underlying mechanism in SARS-CoV-2 infection linked to the blood group. The study cohort consisted of 20-64-year-old subjects, without comorbidities, from both sexes, who were COVID-19 vaccinated with previous or no infection history. Whole blood samples, collected at A.O.R.N. Sant'Anna and San Sebastiano Hospital (Campania Region), were processed by multiparametric cytofluorimetric assay, to characterize CD4+ helper and CD8+ cytotoxic T cell CD3+ subpopulations. The CD45RA, CCR7, CD27, CD28, CD57 and PD-1 markers were investigated to delineate the peripheral T-cell maturation stages. Differences were detected in ABO blood types in CD3+, CD4+ gated on CD3+, CD8+ and CD8+ gated on CD3+ percentage. These results contribute to identifying a memory cell "identikit" profile in COVID-19 disease, thus leading to a useful tool in precision medicine.

References

Gutcher I, Becher B . APC-derived cytokines and T cell polarization in autoimmune inflammation. J Clin Invest. 2007; 117(5):1119-27. PMC: 1857272. DOI: 10.1172/JCI31720. View

Sallusto F, Lenig D, Forster R, Lipp M, Lanzavecchia A . Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature. 1999; 401(6754):708-12. DOI: 10.1038/44385. View

Vitiello A, Ferrara F, Auti A, Di Domenico M, Boccellino M . Advances in the Omicron variant development. J Intern Med. 2022; 292(1):81-90. PMC: 9115048. DOI: 10.1111/joim.13478. View

Farber D, Netea M, Radbruch A, Rajewsky K, Zinkernagel R . Immunological memory: lessons from the past and a look to the future. Nat Rev Immunol. 2016; 16(2):124-8. DOI: 10.1038/nri.2016.13. View

Hoseinnezhad T, Soltani N, Ziarati S, Behboudi E, Mousavi M . The role of HLA genetic variants in COVID-19 susceptibility, severity, and mortality: A global review. J Clin Lab Anal. 2024; 38(1-2):e25005. PMC: 10829690. DOI: 10.1002/jcla.25005. View

Godfrey D, MacDonald H, Kronenberg M, Smyth M, Van Kaer L . NKT cells: what's in a name?. Nat Rev Immunol. 2004; 4(3):231-7. DOI: 10.1038/nri1309. View

Hthelee L, Sadooghiasl A, Kermanshahi S . Moral distress and moral courage among Iraqi nurses during the COVID-19 pandemic: a cross-sectional study. J Med Ethics Hist Med. 2024; 16:19. PMC: 10909337. DOI: 10.18502/jmehm.v16i19.14618. View

Wu Y, Feng Z, Li P, Yu Q . Relationship between ABO blood group distribution and clinical characteristics in patients with COVID-19. Clin Chim Acta. 2020; 509:220-223. PMC: 7832938. DOI: 10.1016/j.cca.2020.06.026. View

Tomiyama H, Matsuda T, Takiguchi M . Differentiation of human CD8(+) T cells from a memory to memory/effector phenotype. J Immunol. 2002; 168(11):5538-50. DOI: 10.4049/jimmunol.168.11.5538. View

10.

Li J, Wang X, Chen J, Cai Y, Deng A, Yang M . Association between ABO blood groups and risk of SARS-CoV-2 pneumonia. Br J Haematol. 2020; 190(1):24-27. PMC: 7267665. DOI: 10.1111/bjh.16797. View

11.

Ellinghaus D, Degenhardt F, Bujanda L, Buti M, Albillos A, Invernizzi P . Genomewide Association Study of Severe Covid-19 with Respiratory Failure. N Engl J Med. 2020; 383(16):1522-1534. PMC: 7315890. DOI: 10.1056/NEJMoa2020283. View

12.

Amoroso A, Magistroni P, Vespasiano F, Bella A, Bellino S, Puoti F . HLA and AB0 Polymorphisms May Influence SARS-CoV-2 Infection and COVID-19 Severity. Transplantation. 2020; 105(1):193-200. DOI: 10.1097/TP.0000000000003507. View

13.

Burren O, Rubio Garcia A, Javierre B, Rainbow D, Cairns J, Cooper N . Chromosome contacts in activated T cells identify autoimmune disease candidate genes. Genome Biol. 2017; 18(1):165. PMC: 5584004. DOI: 10.1186/s13059-017-1285-0. View

14.

Batool Z, Durrani S, Tariq S . Association Of Abo And Rh Blood Group Types To Hepatitis B, Hepatitis C, Hiv And Syphilis Infection, A Five Year' Experience In Healthy Blood Donors In A Tertiary Care Hospital. J Ayub Med Coll Abbottabad. 2017; 29(1):90-92. View

15.

Zhao J, Yang Y, Huang H, Li D, Gu D, Lu X . Relationship Between the ABO Blood Group and the Coronavirus Disease 2019 (COVID-19) Susceptibility. Clin Infect Dis. 2020; 73(2):328-331. PMC: 7454371. DOI: 10.1093/cid/ciaa1150. View

16.

Ballini A, Cantore S, Serretiello E, Troiano G, Smimmo A, Dioguardi M . Multiparametric correlation of laboratory biomarkers to multiorgan failure outcome in hospitalized COVID-19 patients: a retrospective observational study. Eur Rev Med Pharmacol Sci. 2023; 27(18):8962-8974. DOI: 10.26355/eurrev_202309_33817. View

17.

Pannone G, Caponio V, De Stefano I, Ramunno M, Meccariello M, Agostinone A . Lung histopathological findings in COVID-19 disease - a systematic review. Infect Agent Cancer. 2021; 16(1):34. PMC: 8127295. DOI: 10.1186/s13027-021-00369-0. View

18.

Serretiello E, Ballini A, Smimmo A, Acunzo M, Raimo M, Cantore S . Extracellular Vesicles as a Translational Approach for the Treatment of COVID-19 Disease: An Updated Overview. Viruses. 2023; 15(10). PMC: 10611252. DOI: 10.3390/v15101976. View

19.

Jameson J, Havran W . Skin gammadelta T-cell functions in homeostasis and wound healing. Immunol Rev. 2007; 215:114-22. DOI: 10.1111/j.1600-065X.2006.00483.x. View

20.

Bi J, Tian Z . NK Cell Exhaustion. Front Immunol. 2017; 8:760. PMC: 5487399. DOI: 10.3389/fimmu.2017.00760. View