In Vitro Antiviral Activity of Extract Against Human Herpesvirus Type 1

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Jul 27

PMID 39062750

Authors

Marcin Chodkowski

Sylwia Nowak

Martyna Janicka

Marcin Sobczak

Sebastian Granica

Marcin W Banbura

Malgorzata Krzyzowska

Joanna Cymerys

Affiliations

Soon will be listed here.

Abstract

Plant polyphenols possess diverse bioactivities, including antiviral activity against a broad spectrum of viruses. Here, we investigated the virucidal properties of an extract using an in vitro model of human herpesvirus type 1 (HHV-1) infection. Chromatographic analysis indicated that the extract of is rich in various compounds, among which are polyphenols with virucidal activity confirmed in the literature. We found that extract shows an ability to prevent HHV-1 infection by direct inhibition of the virus attachment, penetration, and blocking of infection when used in pretreatment or post-entry treatment. Our results indicate that extract may be a good candidate drug against HHV-1, both as a substance to prevent infection and to treat an already ongoing infection. Our findings illustrate that could be a potential new candidate for clinical consideration in the treatment of HHV-1 infection alone or in combination with other therapeutics.

References

Date A, Destache C . Natural polyphenols: potential in the prevention of sexually transmitted viral infections. Drug Discov Today. 2015; 21(2):333-41. DOI: 10.1016/j.drudis.2015.10.019. View

Gao J, Cao C, Shi M, Hong S, Guo S, Li J . Kaempferol inhibits SARS-CoV-2 invasion by impairing heptad repeats-mediated viral fusion. Phytomedicine. 2023; 118:154942. PMC: 10289257. DOI: 10.1016/j.phymed.2023.154942. View

Namvar L, Olofsson S, Bergstrom T, Lindh M . Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2. J Clin Microbiol. 2005; 43(5):2058-64. PMC: 1153722. DOI: 10.1128/JCM.43.5.2058-2064.2005. View

Zhao C, Wang F, Tang B, Han J, Li X, Lian G . Anti-inflammatory effects of kaempferol-3-O-rhamnoside on HSV-1 encephalitis in vivo and in vitro. Neurosci Lett. 2021; 765:136172. DOI: 10.1016/j.neulet.2021.136172. View

Durazzo A, Lucarini M, Souto E, Cicala C, Caiazzo E, Izzo A . Polyphenols: A concise overview on the chemistry, occurrence, and human health. Phytother Res. 2019; 33(9):2221-2243. DOI: 10.1002/ptr.6419. View

Ikeda K, Tsujimoto K, Uozaki M, Nishide M, Suzuki Y, Koyama A . Inhibition of multiplication of herpes simplex virus by caffeic acid. Int J Mol Med. 2011; 28(4):595-8. DOI: 10.3892/ijmm.2011.739. View

Chiang L, Ng L, Cheng P, Chiang W, Lin C . Antiviral activities of extracts and selected pure constituents of Ocimum basilicum. Clin Exp Pharmacol Physiol. 2005; 32(10):811-6. DOI: 10.1111/j.1440-1681.2005.04270.x. View

Sudomova M, Berchova-Bimova K, Mazurakova A, Samec D, Kubatka P, Hassan S . Flavonoids Target Human Herpesviruses That Infect the Nervous System: Mechanisms of Action and Therapeutic Insights. Viruses. 2022; 14(3). PMC: 8949561. DOI: 10.3390/v14030592. View

Chiow K, Phoon M, Putti T, Tan B, Chow V . Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection. Asian Pac J Trop Med. 2016; 9(1):1-7. PMC: 7104935. DOI: 10.1016/j.apjtm.2015.12.002. View

10.

Cryer M, Lane K, Greer M, Cates R, Burt S, Andrus M . Isolation and identification of compounds from Kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity. Pharm Biol. 2017; 55(1):1586-1591. PMC: 6130675. DOI: 10.1080/13880209.2017.1310907. View

11.

Boxall S, Kadu N, Dever L, Knerova J, Waller J, Gould P . PPC1 Is Essential for Crassulacean Acid Metabolism and the Regulation of Core Circadian Clock and Guard Cell Signaling Genes. Plant Cell. 2020; 32(4):1136-1160. PMC: 7145507. DOI: 10.1105/tpc.19.00481. View

12.

Montenegro-Landivar M, Tapia-Quiros P, Vecino X, Reig M, Valderrama C, Granados M . Polyphenols and their potential role to fight viral diseases: An overview. Sci Total Environ. 2021; 801:149719. PMC: 8373592. DOI: 10.1016/j.scitotenv.2021.149719. View

13.

Sochocka M, Sobczynski M, Ochnik M, Zwolinska K, Leszek J . Hampering Herpesviruses HHV-1 and HHV-2 Infection by Extract of (EGb) and Its Phytochemical Constituents. Front Microbiol. 2019; 10:2367. PMC: 6803450. DOI: 10.3389/fmicb.2019.02367. View

14.

Carvalho O, Botelho C, Ferreira C, Ferreira H, Santos M, Diaz M . In vitro inhibition of canine distemper virus by flavonoids and phenolic acids: implications of structural differences for antiviral design. Res Vet Sci. 2013; 95(2):717-24. DOI: 10.1016/j.rvsc.2013.04.013. View

15.

Hassan S, Sudomova M, Mazurakova A, Kubatka P . Insights into Antiviral Properties and Molecular Mechanisms of Non-Flavonoid Polyphenols against Human Herpesviruses. Int J Mol Sci. 2022; 23(22). PMC: 9693824. DOI: 10.3390/ijms232213891. View

16.

Krzyzowska M, Chodkowski M, Janicka M, Dmowska D, Tomaszewska E, Ranoszek-Soliwoda K . Lactoferrin-Functionalized Noble Metal Nanoparticles as New Antivirals for HSV-2 Infection. Microorganisms. 2022; 10(1). PMC: 8780146. DOI: 10.3390/microorganisms10010110. View

17.

Urmenyi F, Saraiva G, Casanova L, Matos A, de Magalhaes Camargo L, Romanos M . Anti-HSV-1 and HSV-2 Flavonoids and a New Kaempferol Triglycoside from the Medicinal Plant Kalanchoe daigremontiana. Chem Biodivers. 2016; 13(12):1707-1714. DOI: 10.1002/cbdv.201600127. View

18.

Naithani R, Huma L, Holland L, Shukla D, McCormick D, Mehta R . Antiviral activity of phytochemicals: a comprehensive review. Mini Rev Med Chem. 2008; 8(11):1106-33. DOI: 10.2174/138955708785909943. View

19.

James C, Harfouche M, Welton N, Turner K, Abu-Raddad L, Gottlieb S . Herpes simplex virus: global infection prevalence and incidence estimates, 2016. Bull World Health Organ. 2020; 98(5):315-329. PMC: 7265941. DOI: 10.2471/BLT.19.237149. View

20.

Stefanowicz-Hajduk J, Hering A, Gucwa M, Sztormowska-Achranowicz K, Kowalczyk M, Soluch A . An In Vitro Anticancer, Antioxidant, and Phytochemical Study on Water Extract of Raym.-Hamet and H. Perrier. Molecules. 2022; 27(7). PMC: 9000682. DOI: 10.3390/molecules27072280. View