Study of Periodontal Bacteria in Diabetic Wistar Rats: Assessing the Anti-Inflammatory Effects of Carvacrol and Magnolol Hydrogels

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2024 Jul 27

PMID 39062018

Authors

Georgiana Ioana Potra Cicalau

Olivia Andreea Marcu

Timea Claudia Ghitea

Gabriela Ciavoi

Raluca Cristina Iurcov

Corina Beiusanu

Daniela Florina Trifan

Laura Gratiela Vicas

Mariana Ganea

Affiliations

Soon will be listed here.

Abstract

Periodontal disease and diabetes often co-occur; both are characterized by chronic inflammation. This study aimed to investigate the anti-inflammatory effects of carvacrol and magnolol when incorporated into a periodontal hydrogel and topically applied to Wistar rats with diabetes-associated periodontal disease. Forty male albino Wistar rats were divided into four groups: PD (induced diabetes and periodontitis), PDC (induced diabetes and periodontitis treated with carvacrol), PDM (induced diabetes and periodontitis treated with magnolol), and PDCM (induced diabetes and periodontitis treated with both carvacrol and magnolol). Post treatment, gingival tissue samples were collected to measure levels of the pro-inflammatory cytokines IL-6 and TNF-α. The PDCM group exhibited significantly lower levels of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) compared to the PD group. The combined application of a periodontal hydrogel containing carvacrol and magnolol may significantly reduce gingival inflammation in rats with diabetes-associated periodontal disease.

Citing Articles

Development and Evaluation of Gelatin-Based Gummy Jellies Enriched with Oregano Oil: Impact on Functional Properties and Controlled Release.

Ganea M, Georgiana Ioana P, Ghitea T, Stefan L, Groza F, Frent O Foods. 2025; 14(3).

PMID: 39942072 PMC: 11817771. DOI: 10.3390/foods14030479.

A Natural Approach to the Prevention and Treatment of Gingivitis and Periodontitis: A Review of Pomegranate's Bioactive Properties.

Potra Cicalau G, Vicas L, Ciavoi G, Ghitea T, Csaba N, Cristea R Life (Basel). 2024; 14(10).

PMID: 39459598 PMC: 11509765. DOI: 10.3390/life14101298.

References

Ghitea T . Correlation of Periodontal Bacteria with Chronic Inflammation Present in Patients with Metabolic Syndrome. Biomedicines. 2021; 9(11). PMC: 8615403. DOI: 10.3390/biomedicines9111709. View

Kuo P, Hung T, Lin C, Hsiao H, Fu M, Hong P . Carvacrol Ameliorates Ligation-Induced Periodontitis in Rats. J Periodontol. 2017; 88(7):e120-e128. DOI: 10.1902/jop.2017.160618. View

Rat L, Moldovan A, Trifan D, Matis L, Murvai G, Maris L . Can the Correlation of Periodontopathies with Gastrointestinal Diseases Be Used as Indicators in Severe Colorectal Diseases?. Biomedicines. 2023; 11(2). PMC: 9953596. DOI: 10.3390/biomedicines11020402. View

Akbarzadeh A, Norouzian D, Mehrabi M, Jamshidi S, Farhangi A, Verdi A . Induction of diabetes by Streptozotocin in rats. Indian J Clin Biochem. 2012; 22(2):60-4. PMC: 3453807. DOI: 10.1007/BF02913315. View

Calapkorur M, Alkan B, Tasdemir Z, Akcali Y, Saatci E . Association of peripheral arterial disease with periodontal disease: analysis of inflammatory cytokines and an acute phase protein in gingival crevicular fluid and serum. J Periodontal Res. 2016; 52(3):532-539. DOI: 10.1111/jre.12419. View

Szalabska-Rapala K, Borymska W, Kaczmarczyk-Sedlak I . Effectiveness of Magnolol, a Lignan from Magnolia Bark, in Diabetes, Its Complications and Comorbidities-A Review. Int J Mol Sci. 2021; 22(18). PMC: 8467064. DOI: 10.3390/ijms221810050. View

Hassan S, Abbas S, Muhammad I, Wu J, Yan S, Ali F . Metals-triggered compound CDPDP exhibits anti-arthritic behavior by downregulating the inflammatory cytokines, and modulating the oxidative storm in mice models with extensive ADMET, docking and simulation studies. Front Pharmacol. 2022; 13:1053744. PMC: 9727203. DOI: 10.3389/fphar.2022.1053744. View

Teixeira A, Freire J, de Sousa L, Parente A, de Sousa N, Arriaga A . L. Extract Decreases Inflammation, Oxidative Stress, and Alveolar Bone Loss in an Experimental Periodontitis Rat Model. Front Physiol. 2017; 8:988. PMC: 5717945. DOI: 10.3389/fphys.2017.00988. View

Rotariu D, Babes E, Tit D, Moisi M, Bustea C, Stoicescu M . Oxidative stress - Complex pathological issues concerning the hallmark of cardiovascular and metabolic disorders. Biomed Pharmacother. 2022; 152:113238. DOI: 10.1016/j.biopha.2022.113238. View

10.

Duarte P, Tezolin K, Figueiredo L, Feres M, Bastos M . Microbial profile of ligature-induced periodontitis in rats. Arch Oral Biol. 2009; 55(2):142-7. DOI: 10.1016/j.archoralbio.2009.10.006. View

11.

Haddad J . Redox regulation of pro-inflammatory cytokines and IkappaB-alpha/NF-kappaB nuclear translocation and activation. Biochem Biophys Res Commun. 2002; 296(4):847-56. DOI: 10.1016/s0006-291x(02)00947-6. View

12.

Fachini-Queiroz F, Kummer R, Estevao-Silva C, de Barros Carvalho M, Cunha J, Grespan R . Effects of Thymol and Carvacrol, Constituents of Thymus vulgaris L. Essential Oil, on the Inflammatory Response. Evid Based Complement Alternat Med. 2012; 2012:657026. PMC: 3418667. DOI: 10.1155/2012/657026. View

13.

Cid-Perez T, Avila-Sosa R, Ochoa-Velasco C, Rivera-Chavira B, Nevarez-Moorillon G . Antioxidant and Antimicrobial Activity of Mexican Oregano () Essential Oil, Hydrosol and Extracts from Waste Solid Residues. Plants (Basel). 2019; 8(1). PMC: 6359612. DOI: 10.3390/plants8010022. View

14.

Yang B, Xu Y, Yu S, Huang Y, Lu L, Liang X . Anti-angiogenic and anti-inflammatory effect of Magnolol in the oxygen-induced retinopathy model. Inflamm Res. 2015; 65(1):81-93. DOI: 10.1007/s00011-015-0894-x. View

15.

Hotamisligil G . Inflammation, metaflammation and immunometabolic disorders. Nature. 2017; 542(7640):177-185. DOI: 10.1038/nature21363. View

16.

Ribeiro D, Freire J, Teixeira A, do Val D, de Freitas A, Fernandes Gomes F . Tocoyena sellowiana extract decreases bone loss in an experimental model of periodontitis in rats: Putative role for cyclooxygenase-2 and IL-1? inhibition. Biomed Pharmacother. 2018; 98:863-872. DOI: 10.1016/j.biopha.2018.01.011. View

17.

Klein A, Carstens M, Carstens E . Eugenol and carvacrol induce temporally desensitizing patterns of oral irritation and enhance innocuous warmth and noxious heat sensation on the tongue. Pain. 2013; 154(10):2078-2087. PMC: 3778037. DOI: 10.1016/j.pain.2013.06.025. View

18.

Andre P, Laugerette F, Feart C . Metabolic Endotoxemia: A Potential Underlying Mechanism of the Relationship between Dietary Fat Intake and Risk for Cognitive Impairments in Humans?. Nutrients. 2019; 11(8). PMC: 6722750. DOI: 10.3390/nu11081887. View

19.

Vargas-Sanchez P, Moro M, Dos Santos F, Anbinder A, Kreich E, Moraes R . Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal model. J Appl Oral Sci. 2017; 25(5):490-497. PMC: 5804385. DOI: 10.1590/1678-7757-2016-0517. View

20.

Acar B, Gumus E, Ozcan-Bulut S, Ozsin-Ozler C, Boyraz M, Tan C . Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case-control study. J Periodontol. 2021; 93(7):1048-1059. DOI: 10.1002/JPER.21-0514. View