GhSBI1, a CUP-SHAPED COTYLEDON 2 Homologue, Modulates Branch Internode Elongation in Cotton

Overview

Journal Plant Biotechnol J

Specialties Biology
Biotechnology

Date 2024 Jul 26

PMID 39058556

Authors

Weiping Zhong

Lanxin Wu

Yan Li

Xiaxuan Li

Junyi Wang

Jingwen Pan

Shouhong Zhu

Shentao Fang

Jinbo Yao

Yongshan Zhang

Wei Chen

Affiliations

Soon will be listed here.

Abstract

Branch length is an important plant architecture trait in cotton (Gossypium) breeding. Development of cultivars with short branch has been proposed as a main object to enhance cotton yield potential, because they are suitable for high planting density. Here, we report the molecular cloning and characterization of a semi-dominant quantitative trait locus, Short Branch Internode 1(GhSBI1), which encodes a NAC transcription factor homologous to CUP-SHAPED COTYLEDON 2 (CUC2) and is regulated by microRNA ghr-miR164. We demonstrate that a point mutation found in sbi1 mutants perturbs ghr-miR164-directed regulation of GhSBI1, resulting in an increased expression level of GhSBI1. The sbi1 mutant was sensitive to exogenous gibberellic acid (GA) treatments. Overexpression of GhSBI1 inhibited branch internode elongation and led to the decreased levels of bioactive GAs. In addition, gene knockout analysis showed that GhSBI1 is required for the maintenance of the boundaries of multiple tissues in cotton. Transcriptome analysis revealed that overexpression of GhSBI1 affects the expression of plant hormone signalling-, axillary meristems initiation-, and abiotic stress response-related genes. GhSBI1 interacted with GAIs, the DELLA repressors of GA signalling. GhSBI1 represses expression of GA signalling- and cell elongation-related genes by directly targeting their promoters. Our work thus provides new insights into the molecular mechanisms for branch length and paves the way for the development of elite cultivars with suitable plant architecture in cotton.

References

Weir I, Lu J, Cook H, Causier B, Schwarz-Sommer Z, Davies B . CUPULIFORMIS establishes lateral organ boundaries in Antirrhinum. Development. 2004; 131(4):915-22. DOI: 10.1242/dev.00993. View

Kierzkowski D, Runions A, Vuolo F, Strauss S, Lymbouridou R, Routier-Kierzkowska A . A Growth-Based Framework for Leaf Shape Development and Diversity. Cell. 2019; 177(6):1405-1418.e17. PMC: 6548024. DOI: 10.1016/j.cell.2019.05.011. View

Tian Z, Zhang Y, Zhu L, Jiang B, Wang H, Gao R . Strigolactones act downstream of gibberellins to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum). Plant Cell. 2022; 34(12):4816-4839. PMC: 9709996. DOI: 10.1093/plcell/koac270. View

Aida M, Ishida T, Tasaka M . Shoot apical meristem and cotyledon formation during Arabidopsis embryogenesis: interaction among the CUP-SHAPED COTYLEDON and SHOOT MERISTEMLESS genes. Development. 1999; 126(8):1563-70. DOI: 10.1242/dev.126.8.1563. View

Wu Y, Min L, Wu Z, Yang L, Zhu L, Yang X . Defective pollen wall contributes to male sterility in the male sterile line 1355A of cotton. Sci Rep. 2015; 5:9608. PMC: 4456728. DOI: 10.1038/srep09608. View

Tan H, Man C, Xie Y, Yan J, Chu J, Huang J . A Crucial Role of GA-Regulated Flavonol Biosynthesis in Root Growth of Arabidopsis. Mol Plant. 2019; 12(4):521-537. DOI: 10.1016/j.molp.2018.12.021. View

Caarls L, Elberse J, Awwanah M, Ludwig N, de Vries M, Zeilmaker T . JASMONATE-INDUCED OXYGENASES down-regulate plant immunity by hydroxylation and inactivation of the hormone jasmonic acid. Proc Natl Acad Sci U S A. 2017; 114(24):6388-6393. PMC: 5474790. DOI: 10.1073/pnas.1701101114. View

Aida M, Ishida T, Fukaki H, Fujisawa H, Tasaka M . Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant. Plant Cell. 1997; 9(6):841-57. PMC: 156962. DOI: 10.1105/tpc.9.6.841. View

Heinrich M, Hettenhausen C, Lange T, Wunsche H, Fang J, Baldwin I . High levels of jasmonic acid antagonize the biosynthesis of gibberellins and inhibit the growth of Nicotiana attenuata stems. Plant J. 2012; 73(4):591-606. DOI: 10.1111/tpj.12058. View

10.

Itoh H, Ueguchi-Tanaka M, Sato Y, Ashikari M, Matsuoka M . The gibberellin signaling pathway is regulated by the appearance and disappearance of SLENDER RICE1 in nuclei. Plant Cell. 2002; 14(1):57-70. PMC: 150551. DOI: 10.1105/tpc.010319. View

11.

Feng F, Mei H, Fan P, Li Y, Xu X, Wei H . Dynamic transcriptome and phytohormone profiling along the time of light exposure in the mesocotyl of rice seedling. Sci Rep. 2017; 7(1):11961. PMC: 5607350. DOI: 10.1038/s41598-017-12326-2. View

12.

Jiang D, Chen W, Dong J, Li J, Yang F, Wu Z . Overexpression of miR164b-resistant OsNAC2 improves plant architecture and grain yield in rice. J Exp Bot. 2018; 69(7):1533-1543. PMC: 5888996. DOI: 10.1093/jxb/ery017. View

13.

Mao C, He J, Liu L, Deng Q, Yao X, Liu C . OsNAC2 integrates auxin and cytokinin pathways to modulate rice root development. Plant Biotechnol J. 2019; 18(2):429-442. PMC: 6953191. DOI: 10.1111/pbi.13209. View

14.

Maugarny-Cales A, Cortizo M, Adroher B, Borrega N, Goncalves B, Brunoud G . Dissecting the pathways coordinating patterning and growth by plant boundary domains. PLoS Genet. 2019; 15(1):e1007913. PMC: 6363235. DOI: 10.1371/journal.pgen.1007913. View

15.

Wang J, Bao J, Zhou B, Li M, Li X, Jin J . The osa-miR164 target OsCUC1 functions redundantly with OsCUC3 in controlling rice meristem/organ boundary specification. New Phytol. 2020; 229(3):1566-1581. PMC: 7821251. DOI: 10.1111/nph.16939. View

16.

Hou Q, Zhao W, Lu L, Wang L, Zhang T, Hu B . Overexpression of HLH4 Inhibits Cell Elongation and Anthocyanin Biosynthesis in . Cells. 2022; 11(7). PMC: 8997993. DOI: 10.3390/cells11071087. View

17.

Mao C, Lu S, Lv B, Zhang B, Shen J, He J . A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis. Plant Physiol. 2017; 174(3):1747-1763. PMC: 5490923. DOI: 10.1104/pp.17.00542. View

18.

Falcone Ferreyra M, Rius S, Casati P . Flavonoids: biosynthesis, biological functions, and biotechnological applications. Front Plant Sci. 2012; 3:222. PMC: 3460232. DOI: 10.3389/fpls.2012.00222. View

19.

Hu H, Wang M, Ding Y, Zhu S, Zhao G, Tu L . Transcriptomic repertoires depict the initiation of lint and fuzz fibres in cotton (Gossypium hirsutum L.). Plant Biotechnol J. 2017; 16(5):1002-1012. PMC: 5902776. DOI: 10.1111/pbi.12844. View

20.

Kazan K, Manners J . JAZ repressors and the orchestration of phytohormone crosstalk. Trends Plant Sci. 2011; 17(1):22-31. DOI: 10.1016/j.tplants.2011.10.006. View