Protective Effects of Polysaccharide Composite Microcapsules on the Survival of During Simulated Gastrointestinal Digestion and Heat Treatment

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2024 Jul 26

PMID 39057417

Authors

Honghui Guo

Yelin Zhou

Quanling Xie

Hui Chen

Mingen Zhang

Lei Yu

Guangyu Yan

Yan Chen

Xueliang Lin

Yiping Zhang

Zhuan Hong

Affiliations

Soon will be listed here.

Abstract

To improve probiotics' survivability during gastrointestinal digestion and heat treatment, was microencapsulated by spray-drying using polysaccharide/sodium caseinate/gelatin (LJP/SC/GE) composites. Thermogravimetry and differential scanning calorimetry results revealed that the denaturation of LJP/SC/GE microcapsules requires higher thermal energy than that of SC/GE microcapsules, and the addition of LJP may improve thermal stability. Zeta potential measurements indicated that, at low pH of the gastric fluid, the negatively charged LJP attracted the positively charged SC/GE, helping to maintain an intact microstructure without disintegration. The encapsulation efficiency of -loaded LJP/SC/GE microcapsules reached about 93.4%, and the survival rate was 46.9% in simulated gastric fluid (SGF) for 2 h and 96.0% in simulated intestinal fluid (SIF) for 2 h. In vitro release experiments showed that the LJP/SC/GE microcapsules could protect the viability of in SGF and release probiotics slowly in SIF. The cell survival of LJP/SC/GE microcapsules was significantly improved during the heat treatment compared to SC/GE microcapsules and free cells. LJP/SC/GE microcapsules can increase the survival of by maintaining the lactate dehydrogenase and Na-K-ATPase activity. Overall, this study demonstrates the great potential of LJP/SC/GE microcapsules to protect and deliver probiotics in food and pharmaceutical systems.

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