Modular Polyketide Synthase Ketosynthases Collaborate with Upstream Dehydratases to Install Double Bonds

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2024 Jul 26

PMID 39056119

Authors

Katherine A Ray

Nisha Saif

Adrian T Keatinge-Clay

Affiliations

Soon will be listed here.

Abstract

A VMYH motif was determined to help ketosynthases in polyketide assembly lines select α,β-unsaturated intermediates from an equilibrium mediated by an upstream dehydratase. Alterations of this motif decreased ketosynthase selectivity within a model tetraketide synthase, most significantly when replaced by the TNGQ motif of ketosynthases that accept D-β-hydroxy intermediates.

Citing Articles

Mutagenesis Supports AlphaFold Prediction of How Modular Polyketide Synthase Acyl Carrier Proteins Dock With Downstream Ketosynthases.

Hirsch M, Desai R, Annaswamy S, Keatinge-Clay A Proteins. 2024; 92(12):1375-1384.

PMID: 39078105 PMC: 11543512. DOI: 10.1002/prot.26733.

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