Different Genotypes and Species of Symbiotic Fungi Mediate the Behavioral Response of Invasive Fabricius (Hymenoptera: Siricidae)

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 Jul 26

PMID 39056012

Authors

Ming Wang

Chenglong Gao

QinWang Xu

Ningning Fu

Jiale Li

Lili Ren

Youqing Luo

Affiliations

Soon will be listed here.

Abstract

In northeast China, the invasive woodwasp., , attacks var. Litv and often shares habitat with native . Previous research showed that can utilize the volatiles from its symbiotic fungus ( IGS-BD) to locate host trees. Consequently, symbiotic fungi ( IGS-D and ) carried by may influence the behavioral selection of . This study aimed to investigate the impact of fungal odor sources on 's behavior in laboratory and field experiments. Our observations revealed that female woodwasps exhibited greater attraction toward the fungal volatiles of 14-day-old IGS-D in a "Y"-tube olfactometer and wind tunnel. When woodwasps were released into bolts inoculated separately with three strains in the field, females of exhibited a preference for those bolts pre-inoculated with IGS-BD. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that the volatiles emitted by the two genotypes of were similar yet significantly distinct from those of . Hence, we postulate that the existence of native IGS-D could potentially facilitate the colonization of in scenarios with minimal or no IGS-BD present in the host.

References

Cooperband M, Boroczky K, Hartness A, Jones T, Zylstra K, Tumlinson J . Male-produced pheromone in the European woodwasp, Sirex noctilio. J Chem Ecol. 2012; 38(1):52-62. DOI: 10.1007/s10886-012-0060-7. View

Lu M, Wingfield M, Gillette N, Mori S, Sun J . Complex interactions among host pines and fungi vectored by an invasive bark beetle. New Phytol. 2010; 187(3):859-66. DOI: 10.1111/j.1469-8137.2010.03316.x. View

Slippers B, Hurley B, Wingfield M . Sirex woodwasp: a model for evolving management paradigms of invasive forest pests. Annu Rev Entomol. 2014; 60:601-19. DOI: 10.1146/annurev-ento-010814-021118. View

Masague S, Fernandez P, Devescovi F, Segura D, de la Vega G, Corley J . Oviposition substrate location by the invasive woodwasp Sirex noctilio: the combined effect of chemical cues emitted by its obligate symbiont Amylostereum areolatum and different host-tree species. Pest Manag Sci. 2023; 79(10):3959-3969. DOI: 10.1002/ps.7596. View

Gao C, Ren L, Wang M, Wang Z, Fu N, Wang H . Full-Length Transcriptome Sequencing-Based Analysis of var. in Response to Venom. Insects. 2022; 13(4). PMC: 9029201. DOI: 10.3390/insects13040338. View

Yousuf F, Gurr G, Carnegie A, Bedding R, Bashford R, Gitau C . The bark beetle, Ips grandicollis, disrupts biological control of the woodwasp, Sirex noctilio, via fungal symbiont interactions. FEMS Microbiol Ecol. 2013; 88(1):38-47. DOI: 10.1111/1574-6941.12267. View

Wang L, Ren L, Liu X, Shi J, Wang J, Luo Y . Effects of endophytic fungi in Mongolian pine on the selection behavior of woodwasp (Sirex noctilio) and the growth of its fungal symbiont. Pest Manag Sci. 2018; 75(2):492-505. DOI: 10.1002/ps.5146. View

Sun X, Tao J, Ren L, Shi J, Luo Y . Identification of Sirex noctilio (Hymenoptera: Siricidae) Using a Species-Specific Cytochrome C Oxidase Subunit I PCR Assay. J Econ Entomol. 2016; 109(3):1424-1430. DOI: 10.1093/jee/tow060. View

Hajek A, Nielsen C, Kepler R, Long S, Castrillo L . Fidelity among Sirex woodwasps and their fungal symbionts. Microb Ecol. 2013; 65(3):753-62. PMC: 3622004. DOI: 10.1007/s00248-013-0218-z. View

10.

Castrillo L, Hajek A, Pajares J, Thomsen I, Csoka G, Kenaley S . Multilocus genotyping of Amylostereum spp. associated with Sirex noctilio and other woodwasps from Europe reveal clonal lineage introduced to the US. Fungal Biol. 2015; 119(7):595-604. DOI: 10.1016/j.funbio.2015.03.004. View

11.

Li D, Shi J, Lu M, Ren L, Zhen C, Luo Y . Detection and Identification of the Invasive Sirex noctilio (Hymenoptera: Siricidae) Fungal Symbiont, Amylostereum areolatum (Russulales: Amylostereacea), in China and the Stimulating Effect of Insect Venom on Laccase Production by A. areolatum YQL03. J Econ Entomol. 2015; 108(3):1136-47. DOI: 10.1093/jee/tov072. View

12.

Wang M, Fu N, Gao C, Wang L, Ren L, Luo Y . Multilocus Genotyping and Intergenic Spacer Single Nucleotide Polymorphisms of (Russulales: Amylostereacea) Symbionts of Native and Non-Native Species. J Fungi (Basel). 2021; 7(12). PMC: 8704056. DOI: 10.3390/jof7121065. View

13.

Wang L, Li C, Shi J, Li C, Li J, Ren L . Incidental Fungi in Host Trees Disrupt the Development of Sirex noctilio (Hymenoptera: Siricidae) Symbiotic Fungus and Larvae. J Econ Entomol. 2020; 113(2):832-838. DOI: 10.1093/jee/toz314. View

14.

Wooding A, Wingfield M, Hurley B, Garnas J, de Groot P, Slippers B . Lack of fidelity revealed in an insect-fungal mutualism after invasion. Biol Lett. 2013; 9(4):20130342. PMC: 3730645. DOI: 10.1098/rsbl.2013.0342. View