Investigation of Benzoic Acid and Sorbic Acid Concentrations in Tomato Paste, Pepper Paste, Ketchup, Mayonnaise, and Barbeque Sauce Samples by Headspace Gas Chromatography-mass Spectrometry

Overview

Journal Turk J Chem

Publisher Scientific and Technological Research Council of Turkey

Date 2024 Jul 25

PMID 39050496

Authors

Orhan Destanoglu

Affiliations

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Abstract

In this study, it was aimed at investigating benzoic acid (BA) and sorbic acid (SoA) concentrations in tomato paste, pepper paste, ketchup, mayonnaise, and barbeque sauce samples by a validated static headspace gas chromatography-mass spectrometry (GC-MS) method. Salicylic acid (SalA) was used as internal standard and the measurements were conducted in the wide linear concentration ranges of BA and SoA which were 2.5-5000 and 12.5-5000, respectively. The limit of detections (LODs) were determined to be 1.5 and 4.5 mg/kg while the limit of quantifications (LOQs) were 2.5 and 12.5 mg/kg for BA and SoA, respectively. The average recovery% values of BA and SoA were found to be 98.5% and 98.7% in an open tomato paste sample while these values were 98.7% and 100.3% in a mayonnaise sample, respectively. Accuracy of the proposed method was confirmed by statistically (significance test) evaluating excellent recovery values. In real samples, while the results of the canned tomato pastes and industrial sauce samples were found suitable, BA and SoA were determined in some tomato and pepper paste products sold under the traditional or homemade name although use of the preservatives in the pastes were prohibited. It is vital for public health to prevent adulteration in pastes which is indispensable for Turkish cuisine as well as prevalently consumed in the world. Therefore, the proposed method can be used in food control laboratories due to its reliability and consumption of much less toxic chemical reagents.

References

Ohtsuki T, Sato K, Sugimoto N, Akiyama H, Kawamura Y . Absolute quantitative analysis for sorbic acid in processed foods using proton nuclear magnetic resonance spectroscopy. Anal Chim Acta. 2012; 734:54-61. DOI: 10.1016/j.aca.2012.04.033. View

Ochiai N, Sasamoto K, Takino M, Yamashita S, Daishima S, Heiden A . Simultaneous determination of preservatives in beverages, vinegar, aqueous sauces, and quasi-drug drinks by stir-bar sorptive extraction (SBSE) and thermal desorption GC-MS. Anal Bioanal Chem. 2002; 373(1-2):56-63. DOI: 10.1007/s00216-002-1257-3. View

Hsu S, Hu C, Chiu T . Online dynamic pH junction-sweeping for the determination of benzoic and sorbic acids in food products by capillary electrophoresis. Anal Bioanal Chem. 2013; 406(2):635-41. DOI: 10.1007/s00216-013-7481-1. View

Javanmardi F, Nemati M, Ansarin M, Arefhosseini S . Benzoic and sorbic acid in soft drink, milk, ketchup sauce and bread by dispersive liquid-liquid microextraction coupled with HPLC. Food Addit Contam Part B Surveill. 2014; 8(1):32-9. DOI: 10.1080/19393210.2014.955534. View

Imanulkhan , Setyaningsih W, Rohman A, Palma M . Development and Validation of HPLC-DAD Method for Simultaneous Determination of Seven Food Additives and Caffeine in Powdered Drinks. Foods. 2020; 9(8). PMC: 7466259. DOI: 10.3390/foods9081119. View

Zengin N, Yuzbasioglu D, Unal F, Yilmaz S, Aksoy H . The evaluation of the genotoxicity of two food preservatives: sodium benzoate and potassium benzoate. Food Chem Toxicol. 2010; 49(4):763-9. DOI: 10.1016/j.fct.2010.11.040. View

Timofeeva I, Kanashina D, Stepanova K, Bulatov A . A simple and highly-available microextraction of benzoic and sorbic acids in beverages and soy sauce samples for high performance liquid chromatography with ultraviolet detection. J Chromatogr A. 2018; 1588:1-7. DOI: 10.1016/j.chroma.2018.12.030. View

Sagandykova G, Alimzhanova M, Nurzhanova Y, Kenessov B . Determination of semi-volatile additives in wines using SPME and GC-MS. Food Chem. 2016; 220:162-167. DOI: 10.1016/j.foodchem.2016.09.164. View

Ulca P, Atamer B, Keskin M, Senyuva H . Sorbate and benzoate in Turkish retail foodstuffs. Food Addit Contam Part B Surveill. 2014; 6(3):209-13. DOI: 10.1080/19393210.2013.795609. View

10.

Sugiura J, Nakajima M . Simultaneous determination of nine preservatives in food by liquid chromatography with the aid of coagulant in the clean-up process. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2017; 34(5):695-704. DOI: 10.1080/19440049.2017.1293302. View

11.

Pereira L, Della Betta F, Seraglio S, Schulz M, Nehring P, Gonzaga L . Assessment of Sorbate and Benzoate Content in Mustard, Ketchup and Tomato Sauce by Sub-Minute Capillary Electrophoresis. Food Technol Biotechnol. 2021; 59(3):376-384. PMC: 8542181. DOI: 10.17113/ftb.59.03.21.7095. View

12.

Iwakoshi K, Shiozawa Y, Yamajima Y, Baba I, Monma K, Kobayashi C . Determination of nine preservatives in processed foods using a modified QuEChERS extraction and quantified by HPLC-PDA. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2019; 36(7):1020-1031. DOI: 10.1080/19440049.2019.1615644. View

13.

Tungkijanansin N, Alahmad W, Nhujak T, Varanusupakul P . Simultaneous determination of benzoic acid, sorbic acid, and propionic acid in fermented food by headspace solid-phase microextraction followed by GC-FID. Food Chem. 2020; 329:127161. DOI: 10.1016/j.foodchem.2020.127161. View

14.

Bian Y, Wang Y, Yu J, Zheng S, Qin F, Zhao L . Analysis of six preservatives in beverages using hydrophilic deep eutectic solvent as disperser in dispersive liquid-liquid microextraction based on the solidification of floating organic droplet. J Pharm Biomed Anal. 2021; 195:113889. DOI: 10.1016/j.jpba.2021.113889. View

15.

Zhao Y, Chen X, Yao S, Pan S, Li X, Jin M . Analysis of nine food additives in red wine by ion-suppression reversed-phase high-performance liquid chromatography using trifluoroacetic acid and ammonium acetate as ion-suppressors. Anal Sci. 2012; 28(10):967-71. DOI: 10.2116/analsci.28.967. View

16.

Yu M, Wen R, Jiang L, Huang S, Fang Z, Chen B . Rapid analysis of benzoic acid and vitamin C in beverages by paper spray mass spectrometry. Food Chem. 2018; 268:411-415. DOI: 10.1016/j.foodchem.2018.06.103. View

17.

Destanoglu O . Simultaneous determination of benzoic acid and sorbic acid in non-alcoholic beverages by a validated HS-GC-MS method with reduced waste. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2023; 40(7):812-823. DOI: 10.1080/19440049.2023.2224891. View

18.

Xu J, Chen B, He M, Hu B . Analysis of preservatives with different polarities in beverage samples by dual-phase dual stir bar sorptive extraction combined with high-performance liquid chromatography. J Chromatogr A. 2013; 1278:8-15. DOI: 10.1016/j.chroma.2012.12.061. View

19.

Wei R, Li W, Yang L, Jiang Y, Xie T . Online preconcentration in capillary electrophoresis with contactless conductivity detection for sensitive determination of sorbic and benzoic acids in soy sauce. Talanta. 2011; 83(5):1487-90. DOI: 10.1016/j.talanta.2010.11.036. View