Role of the GRP78-c-Src Signaling Pathway on Osteoblast Differentiation of Periodontal Ligament Fibroblasts Induced by Cyclic Mechanical Stretch

Overview

Journal Hua Xi Kou Qiang Yi Xue Za Zhi

Specialty Dentistry

Date 2024 Jul 25

PMID 39049649

Authors

Jing Hu

Zhihua Cui

Keqiang Huang

Rongjian Su

Song Zhao

Affiliations

Soon will be listed here.

Abstract

Objectives: This study aims to investigate the influence of glucose regulated protein (GRP) 78 on osteoblast differentiation in periodontal ligament fibroblasts (PDLFs) under cyclic mechanical stretch and determine the underlying mechanism.

Methods: FlexCell 5000 cell mechanical device was applied to simulate the stress environment of orthodontic teeth. GRP78High and GRP78Low subpopulation were obtained by flow sorting. Gene transfection was performed to knockdown GRP78 and c-Src expression and overexpress c-Src. Western blot analysis was used to detect the protein expression of Runt-related gene 2 (RUNX2), Osterix, osteocalcin (OCN), and osteopontin (OPN). Immunoprecipitation assay was used to determine the interaction of GRP78 with c-Src. The formation of cellular mineralized nodules was determined by alizarin red staining.

Results: GRP78 was heterogeneously expressed in PDLFs, and GRP78High and GRP78Low subpopulations were obtained by flow sorting. The osteogenic differentiation ability and phosphorylation level of c-Src kinase in the GRP78High subpopulation were significantly increased compared with those in GRP78Low subpopulation after cyclic mechanical stretch (<0.05). GRP78 interacted with c-Src in PDLFs. The overexpression c-Src group showed significantly increased osteogenic differentiation ability than the vector group (<0.05), and the sic-Src group showed significantly decreased osteogenic differentiation ability (<0.05) after cyclic mechanical stretch.

Conclusions: GRP78 upregulates c-Src expression by interacting with c-Src kinase and promotes osteogenic differentiation under cyclic mechanical stretch in PDLFs.

Citing Articles

The effects of fulvic acids and low-level laser therapy on orthodontic retention in rats.

Zhao J, Liu Q, Zhang C, Zhang K, Xin P BMC Oral Health. 2024; 24(1):1155.

PMID: 39343917 PMC: 11440710. DOI: 10.1186/s12903-024-04943-x.

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