Identification of Granatane Alkaloids from (Solanaceae) Using Molecular Networking and Semisynthesis

Overview

Journal J Nat Prod

Date 2024 Jul 22

PMID 39038492

Authors

Quentin Dutertre

Philippe A Guy

Sylvain Sutour

Manuel C Peitsch

Nikolai V Ivanov

Gaetan Glauser

Stephan von Reuss

Affiliations

Soon will be listed here.

Abstract

The Solanaceae plant family contains at least 98 genera and over 2700 species. The genus stands out for its ability to produce pyridine and tropane alkaloids, which are relatively poorly characterized at the phytochemical level. In this study, we analyzed dried leaves of using supercritical CO extraction and ultra-high-pressure liquid chromatography coupled to high-resolution tandem mass spectrometry, followed by feature-based molecular networking. Thirty-one known tropane alkaloids were putatively annotated, and the identity of six (atropine, scopolamine, anisodamine, aposcopolamine, apoatropine, and noratropine) were identified using reference standards. Two new granatane alkaloids connected in the molecular network were highlighted from , and their α-granatane tropate and α-granatane isovalerate structures were unambiguously established by semisynthesis.

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