The Interaction of a Gemini Surfactant with a DNA Quadruplex

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2024 Jul 22

PMID 39035911

Authors

Emily Howard

Lindsay N Little

Emily A Boatwright

Derek Aguilar

Hellen Nembaware

Alexys Ginegaw

Alaina Jordan

Payton Justice

Rafaela Dominguez

Mekdelawit Muleta

Steven Bachofer

Richard D Sheardy

Affiliations

Soon will be listed here.

Abstract

DNA secondary structures are stabilized by mono- and divalent cations. To examine the stability of the DNA quadruplex formed from (TTAGGG), its interaction with a dicationic Gemini surfactant in standard phosphate buffer was investigated. The Gemini surfactant begins to form micelles in buffer at a (critical micelle concentration) of 1.5 mM. In this study, solutions of DNA were prepared in buffer with surfactant concentrations ranging from 0.0 to 3.0 mM, i.e., above and below the of the surfactant. In all samples of DNA and surfactant, a precipitate formed. The fraction of DNA precipitated depends upon both the initial DNA concentration and the initial concentration of the surfactant. In those samples where the DNA did not totally precipitate, the residual DNA assumed a quadruplex conformation. It was determined that two surfactant molecules per DNA phosphate are needed to completely precipitate all of the DNA in a particular sample. An estimated apparent for the DNA:surfactant complex was determined.

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