Dynamics of Inflammatory and Pathological Changes Induced by Single Exposure of Particulate Matter (PM) in Mice: Potential Implications in COPD

Overview

Journal Cell Biochem Biophys

Specialties Biochemistry
Biophysics
Cell Biology

Date 2024 Jul 20

PMID 39031246

Authors

Jitender Chandel

Amarjit S Naura

Affiliations

Soon will be listed here.

Abstract

Chronic Obstructive Pulmonary Disease (COPD) is a progressive disorder of lungs marked by chronic bronchitis and emphysema. Particulate matter (PM), a major component of air pollution has been correlated with COPD incidence. The present work aimed to understand dynamics of cellular/molecular players behind PM-mediated COPD pathogenesis in mice by conducting dose and time-course studies. Single intratracheal exposure of PM at a dose of either 100 or 200 μg induced inflammatory response in lungs at 4 days. Time course studies showed that inflammation once triggered by PM is progressive in nature as reflected by data on BALF inflammatory cells at 7/14 days. Similarly, various cytokines/chemokines (KC/IL-6/TNF-α/IL-1β/G-CSF/MCP-1) peak at either 7 or 14 days. However, inflammation declined sharply at 21 days. Data on LPO/GSH and activities of SOD/Catalase show induction of continuous oxidative stress in lung tissue. Next, enhanced mtROS in the CD11b inflammatory cells confirms the redox imbalance in neutrophils/macrophages. A continuous decline in lung function was observed till 28 days. Further, histological analysis of lung tissues at 28 days confirmed the presence of emphysematous lesions, validating the potency of PM to cause irreversible damage to lungs through complex interplay of various cellular/molecular players which may be exploited as potential preventive/therapeutic targets.

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References

Jeffery P . Comparison of the structural and inflammatory features of COPD and asthma. Giles F. Filley Lecture. Chest. 2000; 117(5 Suppl 1):251S-60S. DOI: 10.1378/chest.117.5_suppl_1.251s. View

McDonough J, Yuan R, Suzuki M, Seyednejad N, Elliott W, Sanchez P . Small-airway obstruction and emphysema in chronic obstructive pulmonary disease. N Engl J Med. 2011; 365(17):1567-75. PMC: 3238466. DOI: 10.1056/NEJMoa1106955. View

Anderson G, Bozinovski S . Acquired somatic mutations in the molecular pathogenesis of COPD. Trends Pharmacol Sci. 2003; 24(2):71-6. DOI: 10.1016/S0165-6147(02)00052-4. View

Breslin E, van der Schans C, Breukink S, Meek P, Mercer K, VOLZ W . Perception of fatigue and quality of life in patients with COPD. Chest. 1998; 114(4):958-64. DOI: 10.1378/chest.114.4.958. View

Shin S, Bai L, Burnett R, Kwong J, Hystad P, van Donkelaar A . Air Pollution as a Risk Factor for Incident Chronic Obstructive Pulmonary Disease and Asthma. A 15-Year Population-based Cohort Study. Am J Respir Crit Care Med. 2020; 203(9):1138-1148. DOI: 10.1164/rccm.201909-1744OC. View

Zhou J, Liu J, Zhou Y, Xu J, Song Q, Peng L . The impact of fine particulate matter on chronic obstructive pulmonary disease deaths in Pudong New Area, Shanghai, during a long period of air quality improvement. Environ Pollut. 2023; 340(Pt 2):122813. DOI: 10.1016/j.envpol.2023.122813. View

Li J, Hu Y, Liu L, Wang Q, Zeng J, Chen C . PM2.5 exposure perturbs lung microbiome and its metabolic profile in mice. Sci Total Environ. 2020; 721:137432. DOI: 10.1016/j.scitotenv.2020.137432. View

Jeong S, Bae S, Shin E, Lee J, Ha J . Ellagic Acid Prevents Particulate Matter-Induced Pulmonary Inflammation and Hyperactivity in Mice: A Pilot Study. Int J Environ Res Public Health. 2023; 20(5). PMC: 10001477. DOI: 10.3390/ijerph20054523. View

Pathak U, Gupta N, Suri J . Risk of COPD due to indoor air pollution from biomass cooking fuel: a systematic review and meta-analysis. Int J Environ Health Res. 2019; 30(1):75-88. DOI: 10.1080/09603123.2019.1575951. View

10.

Doiron D, de Hoogh K, Probst-Hensch N, Fortier I, Cai Y, De Matteis S . Air pollution, lung function and COPD: results from the population-based UK Biobank study. Eur Respir J. 2019; 54(1). DOI: 10.1183/13993003.02140-2018. View

11.

Lee M, Kim H, Lee S, Lee J . Curcumin Ameliorates Particulate Matter-Induced Pulmonary Injury through Bimodal Regulation of Macrophage Inflammation via NF-κB and Nrf2. Int J Mol Sci. 2023; 24(3). PMC: 9915121. DOI: 10.3390/ijms24031858. View

12.

Hogg J, van Eeden S . Pulmonary and systemic response to atmospheric pollution. Respirology. 2009; 14(3):336-46. DOI: 10.1111/j.1440-1843.2009.01497.x. View

13.

Wang X, Lu W, Xia X, Zhu Y, Ge C, Guo X . Selenomethionine mitigate PM2.5-induced cellular senescence in the lung via attenuating inflammatory response mediated by cGAS/STING/NF-κB pathway. Ecotoxicol Environ Saf. 2022; 247:114266. DOI: 10.1016/j.ecoenv.2022.114266. View

14.

Fu H, Liu X, Li W, Zu Y, Zhou F, Shou Q . PM2.5 Exposure Induces Inflammatory Response in Macrophages via the TLR4/COX-2/NF-κB Pathway. Inflammation. 2020; 43(5):1948-1958. DOI: 10.1007/s10753-020-01269-y. View

15.

Wang Y, Liao S, Pan Z, Jiang S, Fan J, Yu S . Hydrogen sulfide alleviates particulate matter-induced emphysema and airway inflammation by suppressing ferroptosis. Free Radic Biol Med. 2022; 186:1-16. DOI: 10.1016/j.freeradbiomed.2022.04.014. View

16.

Xiong R, Jiang W, Li N, Liu B, He R, Wang B . PM2.5-induced lung injury is attenuated in macrophage-specific NLRP3 deficient mice. Ecotoxicol Environ Saf. 2021; 221:112433. DOI: 10.1016/j.ecoenv.2021.112433. View

17.

Guo C, Sun L, Chen X, Zhang D . Oxidative stress, mitochondrial damage and neurodegenerative diseases. Neural Regen Res. 2014; 8(21):2003-14. PMC: 4145906. DOI: 10.3969/j.issn.1673-5374.2013.21.009. View

18.

Gu W, Hou T, Zhou H, Zhu L, Zhu W, Wang Y . Ferroptosis is involved in PM2.5-induced acute nasal epithelial injury via AMPK-mediated autophagy. Int Immunopharmacol. 2023; 115:109658. DOI: 10.1016/j.intimp.2022.109658. View

19.

Wang Y, Duan H, Zhang J, Wang Q, Peng T, Ye X . YAP1 protects against PM2.5-induced lung toxicity by suppressing pyroptosis and ferroptosis. Ecotoxicol Environ Saf. 2023; 253:114708. DOI: 10.1016/j.ecoenv.2023.114708. View

20.

Fan X, Dong T, Yan K, Ci X, Peng L . PM2.5 increases susceptibility to acute exacerbation of COPD via NOX4/Nrf2 redox imbalance-mediated mitophagy. Redox Biol. 2023; 59:102587. PMC: 9813701. DOI: 10.1016/j.redox.2022.102587. View