Anti-inflammatory Potential of Aconitine Produced by Endophytic Fungus Acremonium Alternatum

Overview

Journal World J Microbiol Biotechnol

Publisher Springer

Specialties Biotechnology
Microbiology

Date 2024 Jul 19

PMID 39030384

Authors

Fatima Bhadra

M Vasundhara

Affiliations

Soon will be listed here.

Abstract

Argemone mexicana belonging to family Papaveraceae is a traditional medicinal plant widely utilized by tribal people in India for treating various ailments like skin infections, wounds and inflammation. This plant is very rich in alkaloidal content, which has a great potential in the treatment of anti-inflammatory disorders. Therapeutically promising bioactive molecules are often produced by endophytic fungi associated with medicinal plants. In this investigation, endophytic fungi were isolated from various parts of A. mexicana and screened for alkaloidal content. Among these, one of the fungal isolate, Acremonium alternatum AMEF-5 producing maximum alkaloids showed significant anti-inflammatory activity. Fractionation of this crude fungal extract through column chromatography yielded eight fractions, which were further screened for anti-inflammatory activities. Fraction 3 exhibited significant anti-inflammatory activity by the inhibition of lipoxygenase enzyme (IC 15.2 ± 0.09 µg/ml), scavenging of the nitric oxide radicals (IC 11.38 ± 0.35 µg/ml), protein denaturation (IC 14.93 ± 0.4 µg/ml), trypsin inhibition (IC 12.06 ± 0.64 µg/ml) and HRBC stabilization (IC 11.9 ± 0.22 µg/ml). The bioactive alkaloid in fraction 3 was identified as aconitine which was confirmed by UV, FTIR, HPLC, HRMS, H NMR, and C NMR analysis. This study demonstrates that endophytic fungi serve a potential source for sustainable production of therapeutically important alkaloids.

Citing Articles

Endophytic Fungal Diversity and Its Interaction Mechanism with Medicinal Plants.

Gao Y, Xu Y, Dong Z, Guo Y, Luo J, Wang F Molecules. 2025; 30(5).

PMID: 40076252 PMC: 11902086. DOI: 10.3390/molecules30051028.

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