The State of Play of Rodent Models for the Study of Infection

Overview

Journal J Med Microbiol

Specialty Microbiology

Date 2024 Jul 19

PMID 39028257

Authors

Anais Brosse

Heloise Coullon

Claire Janoir

Severine Pechine

Affiliations

Soon will be listed here.

Abstract

is the most common cause of nosocomial antibiotic-associated diarrhoea and is responsible for a spectrum of diseases characterized by high levels of recurrence and morbidity. In some cases, complications can lead to death. Currently, several types of animal models have been developed to study various aspects of infection (CDI), such as colonization, virulence, transmission and recurrence. These models have also been used to test the role of environmental conditions, such as diet, age and microbiome that modulate infection outcome, and to evaluate several therapeutic strategies. Different rodent models have been used successfully, such as the hamster model and the gnotobiotic and conventional mouse models. These models can be applied to study either the initial CDI infectious process or recurrences. The applications of existing rodent models and their advantages and disadvantages are discussed here.

References

Giordano N, Hastie J, Smith A, Foss E, Gutierrez-Munoz D, Carlson Jr P . Cysteine Desulfurase IscS2 Plays a Role in Oxygen Resistance in Clostridium difficile. Infect Immun. 2018; 86(8). PMC: 6056869. DOI: 10.1128/IAI.00326-18. View

Warren C, van Opstal E, Ballard T, Kennedy A, Wang X, Riggins M . Amixicile, a novel inhibitor of pyruvate: ferredoxin oxidoreductase, shows efficacy against Clostridium difficile in a mouse infection model. Antimicrob Agents Chemother. 2012; 56(8):4103-11. PMC: 3421617. DOI: 10.1128/AAC.00360-12. View

Fletcher J, Erwin S, Lanzas C, Theriot C . Shifts in the Gut Metabolome and Transcriptome throughout Colonization and Infection in a Mouse Model. mSphere. 2018; 3(2). PMC: 5874438. DOI: 10.1128/mSphere.00089-18. View

Yakabe K, Higashi S, Akiyama M, Mori H, Murakami T, Toyoda A . Dietary-protein sources modulate host susceptibility to Clostridioides difficile infection through the gut microbiota. Cell Rep. 2022; 40(11):111332. DOI: 10.1016/j.celrep.2022.111332. View

Onderdonk A, Cisneros R, Bartlett J . Clostridium difficile in gnotobiotic mice. Infect Immun. 1980; 28(1):277-82. PMC: 550923. DOI: 10.1128/iai.28.1.277-282.1980. View

Karyal C, Hughes J, Kelly M, Luckett J, Kaye P, Cockayne A . Colonisation Factor CD0873, an Attractive Oral Vaccine Candidate against . Microorganisms. 2021; 9(2). PMC: 7913071. DOI: 10.3390/microorganisms9020306. View

Foley M, Walker M, Stewart A, OFlaherty S, Gentry E, Patel S . Bile salt hydrolases shape the bile acid landscape and restrict Clostridioides difficile growth in the murine gut. Nat Microbiol. 2023; 8(4):611-628. PMC: 10066039. DOI: 10.1038/s41564-023-01337-7. View

Lesniak N, Schubert A, Sinani H, Schloss P . Clearance of Clostridioides difficile Colonization Is Associated with Antibiotic-Specific Bacterial Changes. mSphere. 2021; 6(3). PMC: 8103992. DOI: 10.1128/mSphere.01238-20. View

Douce G, Goulding D . Refinement of the hamster model of Clostridium difficile disease. Methods Mol Biol. 2010; 646:215-27. DOI: 10.1007/978-1-60327-365-7_14. View

10.

Corthier G, Muller M, Wilkins T, Lyerly D, LHaridon R . Protection against experimental pseudomembranous colitis in gnotobiotic mice by use of monoclonal antibodies against Clostridium difficile toxin A. Infect Immun. 1991; 59(3):1192-5. PMC: 258389. DOI: 10.1128/iai.59.3.1192-1195.1991. View

11.

Small J . Fatal enterocolitis in hamsters given lincomycin hydrochloride. Lab Anim Care. 1968; 18(4):411-20. View

12.

Best E, Freeman J, Wilcox M . Models for the study of Clostridium difficile infection. Gut Microbes. 2012; 3(2):145-67. PMC: 3370947. DOI: 10.4161/gmic.19526. View

13.

Seekatz A, Theriot C, Molloy C, Wozniak K, Bergin I, Young V . Fecal Microbiota Transplantation Eliminates Clostridium difficile in a Murine Model of Relapsing Disease. Infect Immun. 2015; 83(10):3838-46. PMC: 4567621. DOI: 10.1128/IAI.00459-15. View

14.

Zhang B, Cai J, Yu B, Hua Y, Lau C, Kao R . A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile. BMC Infect Dis. 2016; 16(1):596. PMC: 5075199. DOI: 10.1186/s12879-016-1924-1. View

15.

Madan R, Petri Jr W . Immune responses to Clostridium difficile infection. Trends Mol Med. 2012; 18(11):658-66. PMC: 3500589. DOI: 10.1016/j.molmed.2012.09.005. View

16.

Soavelomandroso A, Gaudin F, Hoys S, Nicolas V, Vedantam G, Janoir C . Biofilm Structures in a Mono-Associated Mouse Model of Infection. Front Microbiol. 2017; 8:2086. PMC: 5661025. DOI: 10.3389/fmicb.2017.02086. View

17.

Theriot C, Koumpouras C, Carlson P, Bergin I, Aronoff D, Young V . Cefoperazone-treated mice as an experimental platform to assess differential virulence of Clostridium difficile strains. Gut Microbes. 2011; 2(6):326-34. PMC: 3337121. DOI: 10.4161/gmic.19142. View

18.

Pavao A, Graham M, Trofimuk O, Delaney M, Yeliseyev V, Bry L . Protocol for using negative pressure isolator systems to study BSL-2 organisms in gnotobiotic murine models. STAR Protoc. 2022; 3(1):101211. PMC: 8897582. DOI: 10.1016/j.xpro.2022.101211. View

19.

Merrigan M, Sambol S, Johnson S, Gerding D . Susceptibility of hamsters to human pathogenic Clostridium difficile strain B1 following clindamycin, ampicillin or ceftriaxone administration. Anaerobe. 2006; 9(2):91-5. DOI: 10.1016/S1075-9964(03)00063-5. View

20.

Spigaglia P, Barketi-Klai A, Collignon A, Mastrantonio P, Barbanti F, Rupnik M . Surface-layer (S-layer) of human and animal Clostridium difficile strains and their behaviour in adherence to epithelial cells and intestinal colonization. J Med Microbiol. 2013; 62(Pt 9):1386-1393. DOI: 10.1099/jmm.0.056556-0. View