Screening Expression Libraries with Nonradioactive Immunological Probes

Overview

Journal Gene

Specialty Molecular Biology

Date 1985 Jan 1

PMID 3902574

Citations 5

Authors

S Z Wang

A Esen

Affiliations

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Abstract

An immunological screening method employing protein A-peroxidase which does not require radiolabelled antibodies for detection of Escherichia coli colonies synthesizing foreign proteins in a cDNA expression library is described. The technique is sensitive, simpler and more rapid than the procedures that rely on radiolabelled antibodies and autoradiography.

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