Integrated Transcriptomics of Human Blood Vessels Defines a Spatially Controlled Niche for Early Mesenchymal Progenitor Cells

Overview

Journal Dev Cell

Publisher Cell Press

Specialties Cell Biology
Reproductive Medicine

Date 2024 Jul 18

PMID 39025061

Authors

Yiyun Wang

Neelima Thottappillil

Mario Gomez-Salazar

Robert J Tower

Qizhi Qin

Ishbel Camila Del Rosario Alvia

Mingxin Xu

Masnsen Cherief

Ray Cheng

Mary Archer

Shreya Arondekar

Sashank Reddy

Kristen Broderick

Bruno Peault

Aaron W James

Affiliations

Soon will be listed here.

Abstract

Human blood vessel walls show concentric layers, with the outermost tunica adventitia harboring mesenchymal progenitor cells. These progenitor cells maintain vessel homeostasis and provide a robust cell source for cell-based therapies. However, human adventitial stem cell niche has not been studied in detail. Here, using spatial and single-cell transcriptomics, we characterized the phenotype, potential, and microanatomic distribution of human perivascular progenitors. Initially, spatial transcriptomics identified heterogeneity between perivascular layers of arteries and veins and delineated the tunica adventitia into inner and outer layers. From this spatial atlas, we inferred a hierarchy of mesenchymal progenitors dictated by a more primitive cell with a high surface expression of CD201 (PROCR). When isolated from humans and mice, CD201 expression typified a mesodermal committed subset with higher osteogenesis and less proliferation than CD201 cells, with a downstream effect on canonical Wnt signaling through DACT2. CD201 cells also displayed high translational potential for bone tissue generation.

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