Co-immobilization of Amine Dehydrogenase and Glucose Dehydrogenase for the Biosynthesis of (S)-2-aminobutan-1-ol in Continuous Flow

Overview

Journal Bioresour Bioprocess

Specialty Biochemistry

Date 2024 Jul 18

PMID 39023666

Authors

Pengcheng Xie

Jin Lan

Jingshuan Zhou

Zhun Hu

Jiandong Cui

Ge Qu

Bo Yuan

Zhoutong Sun

Affiliations

Soon will be listed here.

Abstract

Reductive amination by amine dehydrogenases is a green and sustainable process that produces only water as the by-product. In this study, a continuous flow process was designed utilizing a packed bed reactor filled with co-immobilized amine dehydrogenase wh84 and glucose dehydrogenase for the highly efficient biocatalytic synthesis of chiral amino alcohols. The immobilized amine dehydrogenase wh84 exhibited better thermo-, pH and solvent stability with high activity recovery. (S)-2-aminobutan-1-ol was produced in up to 99% conversion and 99% ee in the continuous flow processes, and the space-time yields were up to 124.5 g L d. The continuous reactions were also extended to 48 h affording up to 91.8% average conversions. This study showcased the important potential to sustainable production of chiral amino alcohols in continuous flow processes.

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