AlgU Mediates Hyperosmotic Tolerance in SN15-2 by Regulating Membrane Stability, ROS Scavenging, and Osmolyte Synthesis

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2024 Jul 18

PMID 39023265

Authors

Jian Wang

Yaping Wang

Shouquan Lu

Haibo Lou

Xiaobing Wang

Wei Wang

Affiliations

Soon will be listed here.

Abstract

can serve as an agricultural biocontrol agent. often encounters hyperosmotic stress during industrial production and field application. The ability of to withstand hyperosmotic stress is important for its application as a biocontrol agent. AlgU is a global regulator responsible for stress response and biocontrol ability. However, the specific regulatory role of AlgU in the hyperosmotic adaptation of is poorly understood. In this study, we found that the AlgU mutation disrupted the hyperosmotic tolerance of . Many genes and metabolites related to cell envelope formation were significantly downregulated in Δ compared with that in the wild-type (WT) strain under hyperosmotic conditions, and we found that the mutation caused membrane integrity to be compromised and increased membrane permeability. Further experiments revealed that the cell envelope integrity protein TolA, which is regulated by AlgU, contributes to cell membrane stability and osmotic tolerance in . In addition, several genes related to oxidative stress response were significantly downregulated in Δ, and higher levels of intracellular reactive oxygen species were found in Δ. Furthermore, we found that the synthesis of N-acetyl glutaminyl glutamine amide is directly regulated by AlgU and contributes to the hyperosmotic adaptation of . This study revealed the mechanisms of AlgU's participation in osmotic tolerance in , and it provides potential molecular targets for research on the hyperosmotic adaptation of .IMPORTANCEIn this study, we found that the extracytoplasmic function sigma factor AlgU is essential for the survival of under hyperosmotic conditions. We provided evidence supporting the roles of AlgU in influencing cell membrane stability, intracellular reactive oxygen species (ROS) accumulation, and dipeptide N-acetylglutaminylglutamine amide (NAGGN) synthesis in under hyperosmotic conditions. Our findings revealed the mechanisms of AlgU's participation in hyperosmotic stress tolerance in and they provide potential molecular targets for research on the hyperosmotic adaptation of , which is of value in improving the biocontrol ability of .

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