Exploring the Role of Innate Lymphoid Cells in the Periodontium: Insights into Immunological Dynamics During Orthodontic Tooth Movement

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2024 Jul 18

PMID 39021572

Authors

Eva Pastille

Anna Konermann

Affiliations

Soon will be listed here.

Abstract

Background: The periodontal ligament (PDL) experiences considerable mechanical stresses between teeth and bone, vital for tissue adaptation, especially in orthodontic tooth movement (OTM). While recent research emphasizes the role of innate lymphoid cells (ILCs) in regulating sterile inflammation, their involvement in periodontal tissues during OTM remains largely unexplored.

Methods: In this study, PDL tissues from orthodontic patients ( = 8) were examined using flow cytometry to detect ILC subtypes. Transwell co-culture systems were used to expose PDL cells to mechanical strain, followed by measuring migration and ratios of sorted ILC subtypes. Statistical analyses were conducted using paired Student's t-test, Kruskal-Wallis test, Dunn's post-test and one-way/two-way ANOVA with Tukey's post-test (p≤ 0.05; **, p≤ 0.01; ***, p≤ 0.001).

Results: Our findings demonstrate a significant increase in CD127+ CD161+ ILC frequencies in PDL tissues during OTM, indicating ILC involvement in sterile inflammation induced by orthodontic forces. Co-culture assays show directed migration of ILC subsets towards PDL cells and substantial proliferation and expansion of ILCs.

Conclusions: This study is the first to comprehensively investigate the role of ILCs in sterile inflammation during OTM, revealing their presence and distribution within PDL tissues' innate immune response , and exploring their migratory and proliferative behavior . The results suggest a crosstalk between ILCs and PDL cells, potentially influencing the inflammatory response and tissue remodeling processes associated with OTM.

References

Mohammadi H, Sharafkandi N, Hemmatzadeh M, Azizi G, Karimi M, Jadidi-Niaragh F . The role of innate lymphoid cells in health and disease. J Cell Physiol. 2017; 233(6):4512-4529. DOI: 10.1002/jcp.26250. View

Kindstedt E, Koskinen Holm C, Palmqvist P, Sjostrom M, Lejon K, Lundberg P . Innate lymphoid cells are present in gingivitis and periodontitis. J Periodontol. 2018; 90(2):200-207. DOI: 10.1002/JPER.17-0750. View

Thulasidharan M, Vijayaraghavan N, Krishnan V . Heat shock protein 70 and the extent of nuclear damage in periodontal ligament compression side cells with orthodontic force application - An ex-vivo evaluation. Int Orthod. 2021; 19(3):487-493. DOI: 10.1016/j.ortho.2021.04.005. View

Russell S, Walsh P . Sterile inflammation - do innate lymphoid cell subsets play a role?. Front Immunol. 2012; 3:246. PMC: 3413015. DOI: 10.3389/fimmu.2012.00246. View

Nikolaus A, Currey J, Lindtner T, Fleck C, Zaslansky P . Importance of the variable periodontal ligament geometry for whole tooth mechanical function: A validated numerical study. J Mech Behav Biomed Mater. 2016; 67:61-73. DOI: 10.1016/j.jmbbm.2016.11.020. View

Li Y, Jacox L, Little S, Ko C . Orthodontic tooth movement: The biology and clinical implications. Kaohsiung J Med Sci. 2018; 34(4):207-214. DOI: 10.1016/j.kjms.2018.01.007. View

Yan Y, Liu F, Kou X, Liu D, Yang R, Wang X . T Cells Are Required for Orthodontic Tooth Movement. J Dent Res. 2015; 94(10):1463-70. DOI: 10.1177/0022034515595003. View

Korniotis S, Thornley T, Kyriazis P, Theodorou E, Ma L, Li L . Hematopoietic Stem/Progenitor Cell Dependent Participation of Innate Lymphoid Cells in Low-Intensity Sterile Inflammation. Front Immunol. 2018; 9:2007. PMC: 6134892. DOI: 10.3389/fimmu.2018.02007. View

Li C, Liu J, Pan J, Wang Y, Shen L, Xu Y . ILC1s and ILC3s Exhibit Inflammatory Phenotype in Periodontal Ligament of Periodontitis Patients. Front Immunol. 2021; 12:708678. PMC: 8350136. DOI: 10.3389/fimmu.2021.708678. View

10.

Li Y, Zhan Q, Bao M, Yi J, Li Y . Biomechanical and biological responses of periodontium in orthodontic tooth movement: up-date in a new decade. Int J Oral Sci. 2021; 13(1):20. PMC: 8239047. DOI: 10.1038/s41368-021-00125-5. View

11.

Rizzi A, Di Gioacchino M, Gammeri L, Inchingolo R, Chini R, Santilli F . The Emerging Role of Innate Lymphoid Cells (ILCs) and Alarmins in Celiac Disease: An Update on Pathophysiological Insights, Potential Use as Disease Biomarkers, and Therapeutic Implications. Cells. 2023; 12(14). PMC: 10378400. DOI: 10.3390/cells12141910. View

12.

Tsuge A, Noda K, Nakamura Y . Early tissue reaction in the tension zone of PDL during orthodontic tooth movement. Arch Oral Biol. 2016; 65:17-25. DOI: 10.1016/j.archoralbio.2016.01.007. View

13.

Bianchi M . DAMPs, PAMPs and alarmins: all we need to know about danger. J Leukoc Biol. 2006; 81(1):1-5. DOI: 10.1189/jlb.0306164. View

14.

Gasteiger G, Fan X, Dikiy S, Lee S, Rudensky A . Tissue residency of innate lymphoid cells in lymphoid and nonlymphoid organs. Science. 2015; 350(6263):981-5. PMC: 4720139. DOI: 10.1126/science.aac9593. View

15.

Nace G, Evankovich J, Eid R, Tsung A . Dendritic cells and damage-associated molecular patterns: endogenous danger signals linking innate and adaptive immunity. J Innate Immun. 2011; 4(1):6-15. DOI: 10.1159/000334245. View

16.

RYGH P . Ultrastructural vascular changes in pressure zones of rat molar periodontium incident to orthodontic movement. Scand J Dent Res. 1972; 80(4):307-21. DOI: 10.1111/j.1600-0722.1972.tb00296.x. View

17.

Salim C, Muders H, Jager A, Konermann A . Role of chaperone-assisted selective autophagy (CASA) in mechanical stress protection of periodontal ligament cells. J Orofac Orthop. 2021; 83(1):1-12. PMC: 8766363. DOI: 10.1007/s00056-021-00358-3. View

18.

Spits H, Artis D, Colonna M, Diefenbach A, Di Santo J, Eberl G . Innate lymphoid cells--a proposal for uniform nomenclature. Nat Rev Immunol. 2013; 13(2):145-9. DOI: 10.1038/nri3365. View

19.

Vivier E, Artis D, Colonna M, Diefenbach A, Di Santo J, Eberl G . Innate Lymphoid Cells: 10 Years On. Cell. 2018; 174(5):1054-1066. DOI: 10.1016/j.cell.2018.07.017. View

20.

Brown J, Campbell L, Malcolm J, Adrados Planell A, Butcher J, Culshaw S . Enrichment of Innate Lymphoid Cell Populations in Gingival Tissue. J Dent Res. 2018; 97(12):1399-1405. DOI: 10.1177/0022034518782141. View