Is FSH Combined with Equine Chorionic Gonadotropin Able to Modify Lipid Metabolism in Bovine Superstimulated Antral Follicles?

Overview

Journal Anim Reprod

Date 2024 Jul 18

PMID 39021495

Authors

Priscila Helena Santos

Fernanda Fagali Franchi

Sarah Gomes Nunes

Patricia Kubo Fontes

Alan Brunholi Giroto

Fernanda Mani

Anthony Cesar de Souza Castilho

Affiliations

Soon will be listed here.

Abstract

Lipid metabolism is essential for ensuring oocyte maturation and embryo development. β-Oxidized fatty acids (FA) are a potent source of energy for cells, particularly for bovine somatic follicular cells. Superstimulatory protocols using follicle stimulating hormone (FSH) or FSH combined with equine chorionic gonadotropin (eCG) are capable of stimulating the follicular microenvironment and drive the expression of biomarker genes associated with lipid metabolism in the cumulus-oocyte complex (COC) for better embryo development. In this study, we assesed the effects of FSH and FSH/eCG protocols on the expression of genes related to lipid metabolism in bovine granulosa cells (GCs). Further, we measured triglyceride levels in follicular fluid (FF) obtained from both superstimulatd and non-superstimulated cows (synchronized cows). In summary, superstimulation with gonadotropins maintained the TG levels in bovine FF and ensured GCs mRNA abundance of and We, however, found the abundance of mRNA to be lower in GCs obtained from cows subjected to FSH/eCG protocols than synchronized cows. In conclusion, the findings of this study showed that ovarian superstimulation around the preovulatory phase has a mild impact on the lipid metabolism in GCs.

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