Investigation of Mitochondrial Gene Region of Both Eggs from Dogs and Cystic Echinococcosis Isolates Obtained from Sheep and Cattle by Molecular Methods

Overview

Journal Iran J Parasitol

Publisher Tehran University of Medical Sciences

Specialty Parasitology

Date 2024 Jul 16

PMID 39011535

Authors

Rahmi Yildiz

Selahattin Aydemir

Ahmet Galip Halidi

Ahmet Hakan Unlu

Hasan Yilmaz

Affiliations

Soon will be listed here.

Abstract

Background: We aimed to determine the common genotypes in Ağrı, Türkiye and to obtain information on the transmission of this parasite.

Methods: Cystic echinococcosis samples from 100 slaughtered cattle and 100 slaughtered sheep and faecal samples from 200 stray dogs were included in 2021. Collected cyst fluid samples and faces were examined microscopically. DNA was isolated from the germinal membrane of the cysts and from the parasite eggs in the stool samples. The mitochondrial gene region of the parasite was amplified by PCR. Genotypes were determined using the Basic Local Alignment Search Tool (BLAST) after sequence analysis of PCR amplicons.

Results: The highest percentage of cysts was found in the lungs of sheep and the liver of cattle. In addition, 75% of sheep cysts and 25.6% of cattle cysts were fertile. Taenia spp./ spp. eggs were found in 6% of the faeces of 200 dogs examined microscopically. eggs were detected in 4 out of 50 stool samples analysed by PCR. All samples analysed by sequence analysis were identified as s.s. G1 genotype. Sequence comparison revealed revealed one or more-point mutations in different regions of the five samples.

Conclusion: s.s. G1 genotype, known as sheep strain, is common in the Ağrı, Türkiye. The controlled slaughter of livestock, especially sheep, and the avoidance of feeding hydatid cyst organs to dogs, together with public education, were necessary to prevent the spread of the disease.

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