The Prevalence of Spp. and in (Rodentia: Sciuridae) in the Qinghai Tibetan Plateau Area, China

Overview

Journal Iran J Parasitol

Publisher Tehran University of Medical Sciences

Specialty Parasitology

Date 2024 Jul 16

PMID 39011525

Authors

Liqing Ma

Yingna Jian

Guanghua Wang

Qigang Cai

Geping Wang

Xiuping Li

Xueyong Zhang

Panagiotis Karanis

Affiliations

Soon will be listed here.

Abstract

Background: and are well-known important intestinal zoonotic pathogens that can infect various hosts and cause diarrhoeal diseases. We aimed to determine the epidemiological prevalence and molecular characterization of and species in Himalayan marmot (, class Marmota) in the Qinghai Tibetan Plateau Area of Qinghai Province, Northwest China.

Methods: Overall, 243 Himalayan marmot fecal samples were collected in 2017 and in 2019 and a two-step nested PCR technique was performed to amplify the fragments of the SSU rRNA gene of and 18S ribosomal RNA gene of . Molecular characterization of was performed with the primary primers NDIAGF2 and N-DIAGR2, the secondary primers CPB-DIAGF and CPB-DIAGR. Similarly, molecular characterization of was used the first primers Gia2029 and Gia2150c, the secondary primers RH11 and RH4. The positive PCR products were sequenced and the sequences were processed by Clustal Omega and BLAST. Phylogenetic analysis was achieved by NJ method in MEGA.

Results: The infection rate of spp. and . was 4.9% (12/243) and 0.8% (2/243) in . , respectively. spp. are characterized as novel genotypes marmot genotype I (n=3) and marmot genotype II (n=9); . assemblage A (n=2) was found in . .

Conclusion: This is the first report of spp. and . infections in . in Qinghai of Northwest China. The results indicate the existence of species and . infections that may have a potential public health significance.

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