Glutamate Neurotransmission from Leptin Receptor Cells is Required for Typical Puberty and Reproductive Function in Female Mice

The hypothalamic ventral premammillary nucleus (PMv) is a glutamatergic nucleus essential for the metabolic control of reproduction. However, conditional deletion of leptin receptor long form (LepRb) in vesicular glutamate transporter 2 (Vglut2) expressing neurons results in virtually no reproductive deficits. In this study, we determined the role of glutamatergic neurotransmission from leptin responsive PMv neurons on puberty and fertility. We first assessed if stimulation of PMv neurons induces luteinizing hormone (LH) release in fed adult females. We used the stimulatory form of designer receptor exclusively activated by designer drugs (DREADDs) in (LepRb-Cre) mice. We collected blood sequentially before and for 1 hr after intravenous clozapine--oxide injection. LH level increased in animals correctly targeted to the PMv, and LH level was correlated to the number of Fos immunoreactive neurons in the PMv. Next, females with deletion of (Vglut2) in LepRb neurons () showed delayed age of puberty, disrupted estrous cycles, increased gonadotropin-releasing hormone (GnRH) concentration in the axon terminals, and disrupted LH secretion, suggesting impaired GnRH release. To assess if glutamate is required for PMv actions in pubertal development, we generated a Cre-induced reexpression of endogenous LepRb () with concomitant deletion of (Vglut2) mice. Rescue of and deletion of in the PMv was obtained by stereotaxic injection of an adeno-associated virus vector expressing Cre recombinase. Control mice with PMv LepRb rescue showed vaginal opening, follicle maturation, and became pregnant, while ;Vglut2 mice showed no pubertal development. Our results indicate that glutamatergic neurotransmission from leptin sensitive neurons regulates the reproductive axis, and that leptin action on pubertal development via PMv neurons requires Vglut2.