Novel Insights into L. Sprouts: Phytochemical Analysis and Anti-Aging Properties

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2024 Jul 13

PMID 38999009

Authors

Ewelina Rostkowska

Ewa Poleszak

Agata Przekora

Michal Wojcik

Rafal Typek

Katarzyna Wojciechowska

Katarzyna Dos Santos Szewczyk

Affiliations

Soon will be listed here.

Abstract

Skin aging is an inevitable and intricate process instigated, among others, by oxidative stress. The search for natural sources that inhibit this mechanism is a promising approach to preventing skin aging. The purpose of our study was to evaluate the composition of phenolic compounds in the micellar extract of sprouts. The results of a liquid chromatography-mass spectrometry (LC-MS) analysis revealed the presence of thirty-two constituents, including phenolic acids, flavanols, flavan-3-ols, flavanones, isoflavones, and other compounds. Subsequently, the extract was assessed for its antioxidant, anti-inflammatory, anti-collagenase, anti-elastase, anti-tyrosinase, and cytotoxic properties, as well as for the evaluation of collagen synthesis. It was demonstrated that micellar extract from common bean sprouts has strong anti-aging properties. The performed WST-8 (a water-soluble tetrazolium salt) assay revealed that selected concentrations of extract significantly increased proliferation of human dermal fibroblasts compared to the control cells in a dose-dependent manner. A similar tendency was observed with respect to collagen synthesis. Our results suggest that micellar extract from sprouts can be considered a promising anti-aging compound for applications in cosmetic formulations.

References

Lee S, Yu J, Phung H, Lee J, Kim K, Kang K . Potential Anti-Skin Aging Effect of (-)-Catechin Isolated from the Root Bark of var. in Tumor Necrosis Factor-α-Stimulated Normal Human Dermal Fibroblasts. Antioxidants (Basel). 2020; 9(10). PMC: 7601800. DOI: 10.3390/antiox9100981. View

Kumar N, Goel N . Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnol Rep (Amst). 2019; 24:e00370. PMC: 6734135. DOI: 10.1016/j.btre.2019.e00370. View

Hernandez-Saavedra D, Mendoza-Sanchez M, Hernandez-Montiel H, Guzman-Maldonado H, Loarca-Pina G, Salgado L . Cooked common beans (Phaseolus vulgaris) protect against β-cell damage in streptozotocin-induced diabetic rats. Plant Foods Hum Nutr. 2013; 68(2):207-12. DOI: 10.1007/s11130-013-0353-1. View

Oomah B, Corbe A, Balasubramanian P . Antioxidant and anti-inflammatory activities of bean ( Phaseolus vulgaris L.) hulls. J Agric Food Chem. 2010; 58(14):8225-30. DOI: 10.1021/jf1011193. View

Nioi P, McMahon M, Itoh K, Yamamoto M, Hayes J . Identification of a novel Nrf2-regulated antioxidant response element (ARE) in the mouse NAD(P)H:quinone oxidoreductase 1 gene: reassessment of the ARE consensus sequence. Biochem J. 2003; 374(Pt 2):337-48. PMC: 1223621. DOI: 10.1042/BJ20030754. View

Saibabu V, Fatima Z, Khan L, Hameed S . Therapeutic Potential of Dietary Phenolic Acids. Adv Pharmacol Sci. 2015; 2015:823539. PMC: 4579300. DOI: 10.1155/2015/823539. View

Ganesan K, Xu B . Polyphenol-Rich Dry Common Beans (Phaseolus vulgaris L.) and Their Health Benefits. Int J Mol Sci. 2017; 18(11). PMC: 5713300. DOI: 10.3390/ijms18112331. View

Fonseca-Hernandez D, Del Carmen Lugo-Cervantes E, Escobedo-Reyes A, Mojica L . Black Bean ( L.) Polyphenolic Extract Exerts Antioxidant and Antiaging Potential. Molecules. 2021; 26(21). PMC: 8587475. DOI: 10.3390/molecules26216716. View

Ullah A, Ullah N, Nawaz T, Aziz T . Molecular Mechanisms of Sanguinarine in Cancer Prevention and Treatment. Anticancer Agents Med Chem. 2022; 23(7):765-778. DOI: 10.2174/1871520622666220831124321. View

10.

Lopez A, El-Naggar T, Duenas M, Ortega T, Estrella I, Hernandez T . Effect of cooking and germination on phenolic composition and biological properties of dark beans (Phaseolus vulgaris L.). Food Chem. 2012; 138(1):547-55. DOI: 10.1016/j.foodchem.2012.10.107. View

11.

Furst R, Zundorf I . Plant-derived anti-inflammatory compounds: hopes and disappointments regarding the translation of preclinical knowledge into clinical progress. Mediators Inflamm. 2014; 2014:146832. PMC: 4060065. DOI: 10.1155/2014/146832. View

12.

Khan M, Usman M, Nawaz H, Rasheed H, Khan R, Anjum S . Formulation of Phaseolus vulgaris L. cream and its characterization. Pak J Pharm Sci. 2020; 33(2(Supplementary)):815-820. View

13.

Chang T . An updated review of tyrosinase inhibitors. Int J Mol Sci. 2009; 10(6):2440-2475. PMC: 2705500. DOI: 10.3390/ijms10062440. View

14.

Lin L, Harnly J, Pastor-Corrales M, Luthria D . The polyphenolic profiles of common bean ( L.). Food Chem. 2014; 107(1):399-410. PMC: 4276374. DOI: 10.1016/j.foodchem.2007.08.038. View

15.

Ullah A, Razzaq A, Alfaifi M, Elbehairi S, Menaa F, Ullah N . Sanguinarine Attenuates Lung Cancer Progression via Oxidative Stress-induced Cell Apoptosis. Curr Mol Pharmacol. 2024; 17:e18761429269383. DOI: 10.2174/0118761429269383231119062233. View

16.

Amir Aslani B, Ghobadi S . Studies on oxidants and antioxidants with a brief glance at their relevance to the immune system. Life Sci. 2016; 146:163-73. DOI: 10.1016/j.lfs.2016.01.014. View

17.

Gautam R, Srivastava A, Jachak S, Saklani A . Anti-inflammatory, cyclooxygenase (COX)-2, COX-1 inhibitory and antioxidant effects of Dysophylla stellata Benth. Fitoterapia. 2009; 81(1):45-9. DOI: 10.1016/j.fitote.2009.07.004. View

18.

Li T, Chen S, Feng T, Dong J, Li Y, Li H . Rutin protects against aging-related metabolic dysfunction. Food Funct. 2016; 7(2):1147-54. DOI: 10.1039/c5fo01036e. View

19.

Choi S, Lee S, Kim K, Joo D, Shin S, Lee J . Biological effects of rutin on skin aging. Int J Mol Med. 2016; 38(1):357-63. DOI: 10.3892/ijmm.2016.2604. View

20.

Shohag M, Wei Y, Yang X . Changes of folate and other potential health-promoting phytochemicals in legume seeds as affected by germination. J Agric Food Chem. 2012; 60(36):9137-43. DOI: 10.1021/jf302403t. View