Comprehensive Quality Assessment of L. Seeds Via HPTLC, LC-QToF, and Anatomical Investigation

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2024 Jul 13

PMID 38998918

Authors

Nazym Tileuberdi

Kumar Katragunta

Sebastian John Adams

Jennyfer A Aldana-Mejia

Ardak Omarbekova

Bharathi Avula

Ikhlas A Khan

Aknur Turgumbayeva

Samir A Ross

Affiliations

Soon will be listed here.

Abstract

The Brassicaceae family, commonly referred to as cruciferous plants, is globally cultivated and consumed, with the genus being particularly renowned for its functional components. These vegetables are rich sources of nutrients and health-promoting phytochemicals, garnering increased attention in recent years. This study presents a comprehensive microscopic, chromatographic, and spectroscopic characterization of L. seeds from Kazakhstan aimed at elucidating their morphological features and chemical composition. Microscopic analysis revealed distinct localization of flavonoids, total lipids, and alkaloids. High-performance thin-layer chromatography (HPTLC) analysis of seed extracts demonstrated a complex chemical profile with significant quantities of non-polar compounds in the hexane extracts. Additionally, methanolic extracts revealed the presence of diverse chemical compounds, including alkaloids, flavonoids, and glucosinolates. The chemical composition exhibited varietal differences across different species, with L. seeds showing higher concentrations of bioactive compounds. Furthermore, liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QToF-MS) analysis provided insights into the chemical composition, with sinapine isomers, feruloyl, and sinapoyl choline derivatives as major compounds in the seeds. This study contributes to a better understanding of the chemical diversity and quality control methods' approximations of L. seeds, highlighting their importance in functional food and nutraceutical applications.

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