Matrine-loaded Nano-liposome Induces Apoptosis in Human Esophageal-squamous Carcinoma KYSE-150 Cells

Overview

Journal Curr Pharm Des

Publisher Bentham Science Publishers

Date 2024 Jul 12

PMID 38994614

Authors

Kai Zhao

Yun Cai

Faisal Raza

Hajra Zafar

Liang Pan

Xifeng Zheng

Wenjie Xu

Ran Li

Feng Shi

Yongbin Ma

Affiliations

Soon will be listed here.

Abstract

Introduction: Esophageal-squamous Cell Carcinoma (ESCC) is often diagnosed at the middle or late stage, thus requiring more effective therapeutic strategies. Pharmacologically, the anti-tumor activity of the principal active constituent of , matrine (MA), has been explored widely. Notwithstanding, it is significant to nanotechnologically enhance the anti-tumor activity of MA in view of its potential to distribute non-tumor cells.

Methods: Herein, MA-loaded Nano-Liposomes (MNLs) were prepared to enhance the effect of anti-ESCC. The MNL showed a smaller sized particle (25.95 ± 1.02 nm) with a low polydispersed index (PDI = 0.130 ± 0.054), uniform spherical morphology, good solution stability, and encapsulated efficiency (65.55% ± 2.47). Furthermore, we determined the characteristics of KYSE-150 cells by cell viability assay, IC, Mitochondrial Membrane Potential (MMP), Western blot, and apoptotic analysis, which indicated that MNLs down-regulated the cell viability and IC in a concentration-dependent manner and induced a significant change in JC-1 fluorescence from red to green.

Results: The above observations resulted in increased Bax and Caspase-3 levels, coupled with a substantial decrease in Bcl-2 and apoptotic promotion at the advanced stage compared with MA.

Conclusion: Based on these results, MNLs may serve as a more effective and promising therapeutic option for ESCC.

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