Green Nanobiocatalysts: Enhancing Enzyme Immobilization for Industrial and Biomedical Applications

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2024 Jul 12

PMID 38993977

Authors

Doaa S R Khafaga

Ghazala Muteeb

Abdullrahman Elgarawany

Mohammad Aatif

Mohd Farhan

Salma Allam

Batool Abdulhadi Almatar

Mohamed G Radwan

Affiliations

Soon will be listed here.

Abstract

Nanobiocatalysts (NBCs), which merge enzymes with nanomaterials, provide a potent method for improving enzyme durability, efficiency, and recyclability. This review highlights the use of eco-friendly synthesis methods to create sustainable nanomaterials for enzyme transport. We investigate different methods of immobilization, such as adsorption, ionic and covalent bonding, entrapment, and cross-linking, examining their pros and cons. The decreased environmental impact of green-synthesized nanomaterials from plants, bacteria, and fungi is emphasized. The review exhibits the various uses of NBCs in food industry, biofuel production, and bioremediation, showing how they can enhance effectiveness and eco-friendliness. Furthermore, we explore the potential impact of NBCs in biomedicine. In general, green nanobiocatalysts are a notable progression in enzyme technology, leading to environmentally-friendly and effective biocatalytic methods that have important impacts on industrial and biomedical fields.

Citing Articles

Metal-organic frameworks in drug delivery: engineering versatile platforms for therapeutic applications.

Khafaga D, El-Morsy M, Faried H, Diab A, Shehab S, Saleh A RSC Adv. 2024; 14(41):30201-30229.

PMID: 39315019 PMC: 11418013. DOI: 10.1039/d4ra04441j.

References

Chansaenpak K, Kamkaew A, Lisnund S, Prachai P, Ratwirunkit P, Jingpho T . Development of a Sensitive Self-Powered Glucose Biosensor Based on an Enzymatic Biofuel Cell. Biosensors (Basel). 2021; 11(1). PMC: 7825672. DOI: 10.3390/bios11010016. View

Hammed A, Polunin Y, Voronov A, Pryor S . Tubular electrosynthesis of silica-coated magnetite and evaluation of magnetic nanobiocatalyst efficacy during biomass hydrolysis. Bioprocess Biosyst Eng. 2022; 45(8):1311-1318. DOI: 10.1007/s00449-022-02746-4. View

Dadi S, Temur N, Gul O, Yilmaz V, Ocsoy I . In Situ Synthesis of Horseradish Peroxidase Nanoflower@Carbon Nanotube Hybrid Nanobiocatalysts with Greatly Enhanced Catalytic Activity. Langmuir. 2023; 39(13):4819-4828. PMC: 10077815. DOI: 10.1021/acs.langmuir.3c00260. View

Dadi S, Celik C, Ocsoy I . Gallic acid nanoflower immobilized membrane with peroxidase-like activity for m-cresol detection. Sci Rep. 2020; 10(1):16765. PMC: 7542149. DOI: 10.1038/s41598-020-73778-7. View

Jahan N, Rasheed K, Rahman K, Hazafa A, Saleem A, Alamri S . Green inspired synthesis of zinc oxide nanoparticles using (milk thistle) extract and evaluation of their potential pesticidal and phytopathogens activities. PeerJ. 2023; 11:e15743. PMC: 10434149. DOI: 10.7717/peerj.15743. View

Kim J, Grate J, Wang P . Nanobiocatalysis and its potential applications. Trends Biotechnol. 2008; 26(11):639-46. DOI: 10.1016/j.tibtech.2008.07.009. View

Zhang C, You S, Liu Y, Wang C, Yan Q, Qi W . Construction of luffa sponge-based magnetic carbon nanocarriers for laccase immobilization and its application in the removal of bisphenol A. Bioresour Technol. 2020; 305:123085. DOI: 10.1016/j.biortech.2020.123085. View

Betancor L, Luckarift H . Bioinspired enzyme encapsulation for biocatalysis. Trends Biotechnol. 2008; 26(10):566-72. DOI: 10.1016/j.tibtech.2008.06.009. View

Correro M, Sykora S, Corvini P, Shahgaldian P . Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts. Methods Enzymol. 2017; 590:77-91. DOI: 10.1016/bs.mie.2016.12.006. View

10.

Sadeghzadeh S, Ghobadi Nejad Z, Ghasemi S, Khafaji M, Borghei S . Removal of bisphenol A in aqueous solution using magnetic cross-linked laccase aggregates from Trametes hirsuta. Bioresour Technol. 2020; 306:123169. DOI: 10.1016/j.biortech.2020.123169. View

11.

Wang L, Jiang R . Reversible his-tagged enzyme immobilization on functionalized carbon nanotubes as nanoscale biocatalyst. Methods Mol Biol. 2011; 743:95-106. DOI: 10.1007/978-1-61779-132-1_8. View

12.

Schwaminger S, Fehn S, Steegmuller T, Rauwolf S, Lowe H, Pfluger-Grau K . Immobilization of PETase enzymes on magnetic iron oxide nanoparticles for the decomposition of microplastic PET. Nanoscale Adv. 2022; 3(15):4395-4399. PMC: 9417550. DOI: 10.1039/d1na00243k. View

13.

Misson M, Zhang H, Jin B . Nanobiocatalyst advancements and bioprocessing applications. J R Soc Interface. 2014; 12(102):20140891. PMC: 4277080. DOI: 10.1098/rsif.2014.0891. View

14.

Kataria N, Garg V . Green synthesis of FeO nanoparticles loaded sawdust carbon for cadmium (II) removal from water: Regeneration and mechanism. Chemosphere. 2018; 208:818-828. DOI: 10.1016/j.chemosphere.2018.06.022. View

15.

Kost O, Beznos O, Davydova N, Manickam D, Nikolskaya I, Guller A . Superoxide Dismutase 1 Nanozyme for Treatment of Eye Inflammation. Oxid Med Cell Longev. 2015; 2015:5194239. PMC: 4678082. DOI: 10.1155/2016/5194239. View

16.

Inamuddin , Shakeel N, Ahamed M, Kanchi S, Abbas Kashmery H . Green synthesis of ZnO nanoparticles decorated on polyindole functionalized-MCNTs and used as anode material for enzymatic biofuel cell applications. Sci Rep. 2020; 10(1):5052. PMC: 7081323. DOI: 10.1038/s41598-020-61831-4. View

17.

Aslan T, Dadi S, Kafdag O, Temur N, Ildiz N, Ocsoy I . Rational design of EDTA-incorporated nanoflowers as novel and effective endodontic disinfection against biofilms. Odontology. 2023; 112(2):444-452. DOI: 10.1007/s10266-023-00857-2. View

18.

Pandit C, Banerjee S, Pandit S, Lahiri D, Kumar V, Chaubey K . Recent advances and challenges in the utilization of nanomaterials in transesterification for biodiesel production. Heliyon. 2023; 9(4):e15475. PMC: 10147985. DOI: 10.1016/j.heliyon.2023.e15475. View

19.

Singh N, Srivastava G, Talat M, Raghubanshi H, Srivastava O, Kayastha A . Cicer α-galactosidase immobilization onto functionalized graphene nanosheets using response surface method and its applications. Food Chem. 2013; 142:430-8. DOI: 10.1016/j.foodchem.2013.07.079. View

20.

Bilal M, Qamar S, Ashraf S, Rodriguez-Couto S, Iqbal H . Robust nanocarriers to engineer nanobiocatalysts for bioprocessing applications. Adv Colloid Interface Sci. 2021; 293:102438. DOI: 10.1016/j.cis.2021.102438. View