Evaluation of the Effects of Opium on the Expression of and in Wistar Rat Bladder

Overview

Journal Bladder Cancer

Publisher IOS Press

Date 2024 Jul 12

PMID 38993529

Authors

Iman Menbari Oskouie

Leila Zareian Baghdadabad

Rahil Mashhadi

Parisa Zahmatkesh

Akram Mirzaei

Alireza Khajavi

Maryam Noori

Gholamreza Mesbah

Seyed Mohammad Kazem Aghamir

Affiliations

Soon will be listed here.

Abstract

Background: Bladder cancer is a malignancy greatly affected by behavioral habits. The aim of this study was to examine the effect of opium on changes in the expression of and in the bladder tissue of rats.

Method: Thirty six rats were divided into six groups: 24 rats in the addicted group received morphine and opium for 4 months with 12 rats in the control group. Blood testing was done for the evaluation of CBC, MDA, and TAC. The bladder tissue was removed and checked by histopathological examination. All total RNA was extracted, then cDNAs were synthesized and the OCT and gene expressions were evaluated by Real-time PCR.

Results: The mRNA expression level in the opium group of rats was significantly increased compared to the control group (13.5 and 6.8 fold in males and females respectively). Also, in the morphine group, similar augmentation was detected (3.8 and 6.7 fold in males and females respectively). The mRNA over-expression level was seen in the morphine group of both genders as compared to the control group (3.7 and 4.2 fold in male and female respectively) but in the opium group, enhancement of mRNA level was seen only in males (6.6 fold). Opium increases both and expression more than morphine in male rats, but in female rats, is increased more by morphine.

Conclusion: Over expression of and was observed in rats treated with opium and morphine. Increased and expression was seen in opium-treated male rats, but in female rats, was increased more by morphine.

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