A Bifunctional β1-3-galactosyl/-acetylgalactosaminyltransferase (PmNatB) for the Highly Efficient Chemoenzymatic Synthesis of Disaccharides

Overview

Journal Org Biomol Chem

Publisher Royal Society of Chemistry

Specialties Biochemistry
Chemistry

Date 2024 Jul 12

PMID 38993172

Authors

Xiaohong Yang

Bijoyananda Mishra

Hai Yu

Yijun Wei

Xi Chen

Affiliations

Soon will be listed here.

Abstract

Glycosyltransferases are nature's key biocatalysts for the formation of glycosidic bonds. Discovery and characterization of new synthetically useful glycosyltransferases are critical for the development of efficient enzymatic and chemoenzymatic strategies for producing complex carbohydrates and glycoconjugates. Herein we report the identification of PmNatB as a bifunctional single-catalytic-domain glycosyltransferase with both β1-3-galactosyltransferase and β1-3--acetylgalactosaminyltransferase activities. It is a novel glycosyltransferase for constructing structurally diverse GalNAcβ3Galα/βOR and Galβ3GalNAcα/βOR disaccharides in one-pot multienzyme systems with generation of UDP-sugars.

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