Butyrate Induces Higher Host Transcriptional Changes to Inhibit Porcine Epidemic Diarrhea Virus Strain CV777 Infection in Porcine Intestine Epithelial Cells

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2024 Jul 11

PMID 38992629

Authors

Zhen Zhong

Yaqin Zhang

Xuting Zhao

Chunbao Zhou

Shubin Zhu

Jiayun Wu

Affiliations

Soon will be listed here.

Abstract

Newborn piglets' health is seriously threatened by the porcine epidemic diarrhea virus (PEDV), which also has a significant effect on the pig industry. The gut microbiota produces butyrate, an abundant metabolite that modulates intestinal function through many methods to improve immunological and intestinal barrier function. The objective of this investigation was to ascertain how elevated butyrate concentrations impacted the host transcriptional profile of PEDV CV777 strain infection. Our findings showed that higher concentrations of butyrate have a stronger inhibitory effect on PEDV CV777 strain infection. According to RNA-seq data, higher concentrations of butyrate induced more significant transcriptional changes in IPEC-J2 cells, and signaling pathways such as PI3K-AKT may play a role in the inhibition of PEDV CV777 strain by high concentrations of butyrate. Ultimately, we offer a theoretical and experimental framework for future research and development of novel approaches to harness butyrate's antiviral infection properties.

Citing Articles

Identification and mechanism analysis of biomarkers related to butyrate metabolism in COVID-19 patients.

Zhou W, Li H, Zhang J, Liu C, Liu D, Chen X Ann Med. 2025; 57(1):2477301.

PMID: 40074706 PMC: 11905318. DOI: 10.1080/07853890.2025.2477301.

References

Lin H, Li B, Liu M, Zhou H, He K, Fan H . Nonstructural protein 6 of porcine epidemic diarrhea virus induces autophagy to promote viral replication via the PI3K/Akt/mTOR axis. Vet Microbiol. 2020; 244:108684. PMC: 7165116. DOI: 10.1016/j.vetmic.2020.108684. View

Yang Y, Dudoit S, Luu P, Lin D, Peng V, Ngai J . Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation. Nucleic Acids Res. 2002; 30(4):e15. PMC: 100354. DOI: 10.1093/nar/30.4.e15. View

Stevenson G, Hoang H, Schwartz K, Burrough E, Sun D, Madson D . Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences. J Vet Diagn Invest. 2013; 25(5):649-54. DOI: 10.1177/1040638713501675. View

Wang H, Yang L, Qu H, Feng H, Wu S, Bao W . Global Mapping of H3K4 Trimethylation (H3K4me3) and Transcriptome Analysis Reveal Genes Involved in the Response to Epidemic Diarrhea Virus Infections in Pigs. Animals (Basel). 2019; 9(8). PMC: 6719071. DOI: 10.3390/ani9080523. View

Shi X, Zhang Q, Wang J, Zhang Y, Yan Y, Liu Y . Differential expression analysis of mRNAs, lncRNAs, and miRNAs expression profiles and construction of ceRNA networks in PEDV infection. BMC Genomics. 2022; 23(1):586. PMC: 9375197. DOI: 10.1186/s12864-022-08805-0. View

Feng W, Wu Y, Chen G, Fu S, Li B, Huang B . Sodium Butyrate Attenuates Diarrhea in Weaned Piglets and Promotes Tight Junction Protein Expression in Colon in a GPR109A-Dependent Manner. Cell Physiol Biochem. 2018; 47(4):1617-1629. DOI: 10.1159/000490981. View

Song D, Moon H, Kang B . Porcine epidemic diarrhea: a review of current epidemiology and available vaccines. Clin Exp Vaccine Res. 2015; 4(2):166-76. PMC: 4524901. DOI: 10.7774/cevr.2015.4.2.166. View

Pauli G, Ludwig H . Increase of virus yields and releases of Borna disease virus from persistently infected cells. Virus Res. 1985; 2(1):29-33. DOI: 10.1016/0168-1702(85)90057-7. View

Yan H, Ajuwon K . Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway. PLoS One. 2017; 12(6):e0179586. PMC: 5487041. DOI: 10.1371/journal.pone.0179586. View

10.

Li J, Murtaugh M . Dissociation of porcine reproductive and respiratory syndrome virus neutralization from antibodies specific to major envelope protein surface epitopes. Virology. 2012; 433(2):367-76. DOI: 10.1016/j.virol.2012.08.026. View

11.

Koonpaew S, Teeravechyan S, Frantz P, Chailangkarn T, Jongkaewwattana A . PEDV and PDCoV Pathogenesis: The Interplay Between Host Innate Immune Responses and Porcine Enteric Coronaviruses. Front Vet Sci. 2019; 6:34. PMC: 6395401. DOI: 10.3389/fvets.2019.00034. View

12.

Zhang H, Liu Q, Su W, Wang J, Sun Y, Zhang J . Genome-wide analysis of differentially expressed genes and the modulation of PEDV infection in Vero E6 cells. Microb Pathog. 2018; 117:247-254. PMC: 7125602. DOI: 10.1016/j.micpath.2018.02.004. View

13.

Olsen J, Sechelski J . Use of sodium butyrate to enhance production of retroviral vectors expressing CFTR cDNA. Hum Gene Ther. 1995; 6(9):1195-202. DOI: 10.1089/hum.1995.6.9-1195. View

14.

Biagi G, Piva A, Moschini M, Vezzali E, Roth F . Effect of gluconic acid on piglet growth performance, intestinal microflora, and intestinal wall morphology. J Anim Sci. 2006; 84(2):370-8. DOI: 10.2527/2006.842370x. View

15.

Silva Y, Bernardi A, Frozza R . The Role of Short-Chain Fatty Acids From Gut Microbiota in Gut-Brain Communication. Front Endocrinol (Lausanne). 2020; 11:25. PMC: 7005631. DOI: 10.3389/fendo.2020.00025. View

16.

Zhang Y, Chen H, Yu J, Feng R, Chen Z, Zhang X . Comparative transcriptomic analysis of porcine epidemic diarrhea virus epidemic and classical strains in IPEC-J2 cells. Vet Microbiol. 2022; 273:109540. DOI: 10.1016/j.vetmic.2022.109540. View

17.

Morrison D, Preston T . Formation of short chain fatty acids by the gut microbiota and their impact on human metabolism. Gut Microbes. 2016; 7(3):189-200. PMC: 4939913. DOI: 10.1080/19490976.2015.1134082. View

18.

Qin W, Qi X, Xie Y, Wang H, Wu S, Sun M . LncRNA446 Regulates Tight Junctions by Inhibiting the Ubiquitinated Degradation of Alix after Porcine Epidemic Diarrhea Virus Infection. J Virol. 2023; 97(3):e0188422. PMC: 10062151. DOI: 10.1128/jvi.01884-22. View

19.

Trompette A, Gollwitzer E, Pattaroni C, Lopez-Mejia I, Riva E, Pernot J . Dietary Fiber Confers Protection against Flu by Shaping Ly6c Patrolling Monocyte Hematopoiesis and CD8 T Cell Metabolism. Immunity. 2018; 48(5):992-1005.e8. DOI: 10.1016/j.immuni.2018.04.022. View

20.

Hu Z, Li Y, Du H, Ren J, Zheng X, Wei K . Transcriptome analysis reveals modulation of the STAT family in PEDV-infected IPEC-J2 cells. BMC Genomics. 2020; 21(1):891. PMC: 7734901. DOI: 10.1186/s12864-020-07306-2. View