Transgenic Tobacco Plants Overexpressing a Wheat Salt Stress Root Protein (TaSSRP) Exhibit Enhanced Tolerance to Heat Stress

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2024 Jul 11

PMID 38990430

Authors

Mawuli K Azameti

N Tanuja

Satish Kumar

Maniraj Rathinam

Abdul-Wahab M Imoro

P K Singh

Kishor Gaikwad

Rohini Sreevathsa

Monika Dalal

Ajay Arora

Vandna Rai

Jasdeep C Padaria

Affiliations

Soon will be listed here.

Abstract

Background: Heat stress is a detrimental abiotic stress that limits the development of many plant species and is linked to a variety of cellular and physiological problems. Heat stress affects membrane fluidity, which leads to negative effects on cell permeability and ion transport. Research reveals that heat stress causes severe damage to cells and leads to rapid accumulation of reactive oxygen species (ROS), which could cause programmed cell death.

Methods And Results: This current study aimed to validate the role of Triticum aestivum Salt Stress Root Protein (TaSSRP) in plants' tolerance to heat stress by modulating its expression in tobacco plants. The Relative Water Content (RWC), total chlorophyll content, and Membrane Stability Index (MSI) of the seven distinct transgenic lines (T, T, T, T, T, T, and T), increased in response to heat stress. Despite the fact that the same tendency was detected in wild-type (WT) plants, changes in physio-biochemical parameters were greater in transgenic lines than in WT plants. The expression analysis revealed that the transgene TaSSRP expressed from 1.00 to 1.809 folds in different lines in the transgenic tobacco plants. The gene TaSSRP offered resistance to heat stress in Nicotiana tabacum, according to the results of the study.

Conclusion: These findings could help to improve our knowledge and understanding of the mechanism underlying thermotolerance in wheat, and the novel identified gene TaSSRP could be used in generating wheat varieties with enhanced tolerance to heat stress.

Citing Articles

Enhancing wheat resilience: biotechnological advances in combating heat stress and environmental challenges.

Arif M, Haroon M, Nawaz A, Abbas H, Xu R, Li L Plant Mol Biol. 2025; 115(2):41.

PMID: 40057930 DOI: 10.1007/s11103-025-01569-7.

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