Complete Genome Analysis of a Novel Hypovirus in the Phytopathogenic Fungus Monilinia Fructicola

Overview

Journal Arch Virol

Specialty Microbiology

Date 2024 Jul 11

PMID 38990253

Authors

Kang Zhou

Hui Zhou

Zhimin Tang

Affiliations

Soon will be listed here.

Abstract

Monilinia fructicola is one of the most devastating fungal diseases of rosaceous fruit crops, both in the field and postharvest, causing significant yield losses. Here, we report the discovery of a novel positive single-stranded RNA virus, Monilinia fructicola hypovirus 3 (MfHV3), in a strain (hf-1) of the phytopathogenic fungus Monilinia fructicola. The complete genome of MfHV3 is 9259 nucleotides (nt) in length and contains a single large open reading frame (ORF) from nt position 462 to 8411. This ORF encodes a polyprotein with three conserved domains, namely UDP-glycosyltransferase, RNA-dependent RNA polymerase (RdRp), and DEAD-like helicase. The MfHV3 polyprotein shares the highest similarity with Colletotrichum camelliae hypovirus 1. Phylogenetic analysis indicated that MfHV3 clustered with members of the genus Betahypovirus within the family Hypoviridae. Taken together, the results of genomic organization comparisons, amino acid sequence alignments, and phylogenetic analysis convincingly show that MfHV3 is a new member of the genus Betahypovirus, family Hypoviridae.

References

Ghabrial S, Suzuki N . Viruses of plant pathogenic fungi. Annu Rev Phytopathol. 2009; 47:353-84. DOI: 10.1146/annurev-phyto-080508-081932. View

Pearson M, Beever R, Boine B, Arthur K . Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol Plant Pathol. 2009; 10(1):115-28. PMC: 6640375. DOI: 10.1111/j.1364-3703.2008.00503.x. View

Xie J, Jiang D . New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol. 2014; 52:45-68. DOI: 10.1146/annurev-phyto-102313-050222. View

Nuss D . Hypovirulence: mycoviruses at the fungal-plant interface. Nat Rev Microbiol. 2005; 3(8):632-42. DOI: 10.1038/nrmicro1206. View

Yu X, Li B, Fu Y, Xie J, Cheng J, Ghabrial S . Extracellular transmission of a DNA mycovirus and its use as a natural fungicide. Proc Natl Acad Sci U S A. 2013; 110(4):1452-7. PMC: 3557086. DOI: 10.1073/pnas.1213755110. View

Anagnostakis S . Biological control of chestnut blight. Science. 1982; 215(4532):466-71. DOI: 10.1126/science.215.4532.466. View

Nuss D . Biological control of chestnut blight: an example of virus-mediated attenuation of fungal pathogenesis. Microbiol Rev. 1992; 56(4):561-76. PMC: 372888. DOI: 10.1128/mr.56.4.561-576.1992. View

Hu M, Chen Y, Chen S, Liu X, Yin L, Luo C . First Report of Brown Rot of Peach Caused by Monilinia fructicola in Southeastern China. Plant Dis. 2019; 95(2):225. DOI: 10.1094/PDIS-11-10-0779. View

Pellegrino C, Gullino M, Garibaldi A, Spadaro D . First Report of Brown Rot of Stone Fruit Caused by Monilinia fructicola in Italy. Plant Dis. 2019; 93(6):668. DOI: 10.1094/PDIS-93-6-0668A. View

10.

Herrero N, Duenas E, Quesada-Moraga E, Zabalgogeazcoa I . Prevalence and diversity of viruses in the entomopathogenic fungus Beauveria bassiana. Appl Environ Microbiol. 2012; 78(24):8523-30. PMC: 3502908. DOI: 10.1128/AEM.01954-12. View

11.

Bolger A, Lohse M, Usadel B . Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014; 30(15):2114-20. PMC: 4103590. DOI: 10.1093/bioinformatics/btu170. View

12.

Grabherr M, Haas B, Yassour M, Levin J, Thompson D, Amit I . Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011; 29(7):644-52. PMC: 3571712. DOI: 10.1038/nbt.1883. View

13.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

14.

Gong W, Liu H, Zhu X, Zhao S, Cheng J, Zhu H . Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Alternaria solani. Arch Virol. 2021; 166(7):2063-2067. DOI: 10.1007/s00705-021-05105-y. View

15.

Gao F, Anane R, Liu Z, Zi S, Li S, Yang Z . Complete genome sequence of a novel fusarivirus from the phytopathogenic fungus Fusarium sp. Arch Virol. 2023; 168(10):248. DOI: 10.1007/s00705-023-05872-w. View