A Practical Introduction to Holo-omics

Overview

Journal Cell Rep Methods

Specialty Cell Biology

Date 2024 Jul 10

PMID 38986611

Authors

Inaki Odriozola

Jacob A Rasmussen

M Thomas P Gilbert

Morten T Limborg

Antton Alberdi

Affiliations

Soon will be listed here.

Abstract

Holo-omics refers to the joint study of non-targeted molecular data layers from host-microbiota systems or holobionts, which is increasingly employed to disentangle the complex interactions between the elements that compose them. We navigate through the generation, analysis, and integration of omics data, focusing on the commonalities and main differences to generate and analyze the various types of omics, with a special focus on optimizing data generation and integration. We advocate for careful generation and distillation of data, followed by independent exploration and analyses of the single omic layers to obtain a better understanding of the study system, before the integration of multiple omic layers in a final model is attempted. We highlight critical decision points to achieve this aim and flag the main challenges to address complex biological questions regarding the integrative study of host-microbiota relationships.

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