MoRgs3 Functions in Intracellular Reactive Oxygen Species Perception-integrated CAMP Signaling to Promote Appressorium Formation in

Overview

Journal mBio

Publisher American Society for Microbiology

Specialty Microbiology

Date 2024 Jul 9

PMID 38980036

Authors

Ruiming Zhang

Xinyu Liu

Jiayun Xu

Chen Chen

Zhaoxuan Tang

Chengtong Wu

Xinyue Li

Lei Su

Muxing Liu

Leiyun Yang

Gang Li

Haifeng Zhang

Ping Wang

Zhengguang Zhang

Affiliations

Soon will be listed here.

Abstract

Importance: We report that MoRgs3 becomes phosphorylated in an oxidative intracellular environment during the appressorium formation stage. We found that this phosphorylation is carried out by MoNdk1, a nucleoside diphosphate kinase. In addition, this phosphorylation leads to a higher binding affinity between MoRgs3 and MoCrn1, a coronin-like actin-binding protein that was implicated in the endocytic transport of several other RGS proteins of . We further found that the internalization of MoRgs3 is indispensable for its GTPase-activating protein function toward the Gα subunit MoMagA. Importantly, we characterized how such cellular regulatory events coincide with cAMP signaling-regulated appressorium formation and pathogenicity in the blast fungus. Our studies uncovered a novel intracellular reactive oxygen species signal-transducing mechanism in a model pathogenic fungus with important basic and applied implications.

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