Use of Epifluorescence Widefield Deconvolution Microscopy for Imaging and Three-dimensional Rendering of Biofilms and Extracellular Matrix Materials

Overview

Journal Methods Microbiol

Date 2024 Jul 8

PMID 38974073

Authors

Heidi J Smith

Michael J Franklin

Affiliations

Soon will be listed here.

References

Stoodley P, Sauer K, Davies D, Costerton J . Biofilms as complex differentiated communities. Annu Rev Microbiol. 2002; 56:187-209. DOI: 10.1146/annurev.micro.56.012302.160705. View

Chlumsky O, Smith H, Parker A, Brileya K, Wilking J, Purkrtova S . Evaluation of the Antimicrobial Efficacy of -Acetyl-l-Cysteine, Rhamnolipids, and Usnic Acid-Novel Approaches to Fight Food-Borne Pathogens. Int J Mol Sci. 2021; 22(21). PMC: 8583417. DOI: 10.3390/ijms222111307. View

Schlafer S, Meyer R . Confocal microscopy imaging of the biofilm matrix. J Microbiol Methods. 2016; 138:50-59. DOI: 10.1016/j.mimet.2016.03.002. View

Stoodley P, Wilson S, Hall-Stoodley L, Boyle J, Lappin-Scott H, Costerton J . Growth and detachment of cell clusters from mature mixed-species biofilms. Appl Environ Microbiol. 2001; 67(12):5608-13. PMC: 93350. DOI: 10.1128/AEM.67.12.5608-5613.2001. View

Geesey G, Richardson W, Yeomans H, Irvin R, Costerton J . Microscopic examination of natural sessile bacterial populations from an alpine stream. Can J Microbiol. 1977; 23(12):1733-6. DOI: 10.1139/m77-249. View

Parker A, Christen J, Lorenz L, Smith H . Optimal surface estimation and thresholding of confocal microscope images of biofilms using Beer's Law. J Microbiol Methods. 2020; 174:105943. DOI: 10.1016/j.mimet.2020.105943. View

Flemming H, Wingender J . The biofilm matrix. Nat Rev Microbiol. 2010; 8(9):623-33. DOI: 10.1038/nrmicro2415. View

Sauer K, Camper A, Ehrlich G, Costerton J, Davies D . Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm. J Bacteriol. 2002; 184(4):1140-54. PMC: 134825. DOI: 10.1128/jb.184.4.1140-1154.2002. View

Petrova O, Sauer K . Dispersion by Pseudomonas aeruginosa requires an unusual posttranslational modification of BdlA. Proc Natl Acad Sci U S A. 2012; 109(41):16690-5. PMC: 3478618. DOI: 10.1073/pnas.1207832109. View

10.

Costerton J, Lewandowski Z, Caldwell D, Korber D, Lappin-Scott H . Microbial biofilms. Annu Rev Microbiol. 1995; 49:711-45. DOI: 10.1146/annurev.mi.49.100195.003431. View

11.

OToole G, Kolter R . Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol Microbiol. 1998; 30(2):295-304. DOI: 10.1046/j.1365-2958.1998.01062.x. View

12.

OToole G, Kolter R . Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol. 1998; 28(3):449-61. DOI: 10.1046/j.1365-2958.1998.00797.x. View

13.

Franklin M, Chang C, Akiyama T, Bothner B . New Technologies for Studying Biofilms. Microbiol Spectr. 2015; 3(4). PMC: 4821632. DOI: 10.1128/microbiolspec.MB-0016-2014. View

14.

Byrd M, Sadovskaya I, Vinogradov E, Lu H, Sprinkle A, Richardson S . Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production. Mol Microbiol. 2009; 73(4):622-38. PMC: 4409829. DOI: 10.1111/j.1365-2958.2009.06795.x. View

15.

Berne C, Ducret A, Hardy G, Brun Y . Adhesins Involved in Attachment to Abiotic Surfaces by Gram-Negative Bacteria. Microbiol Spectr. 2015; 3(4). PMC: 4566860. DOI: 10.1128/microbiolspec.MB-0018-2015. View

16.

Sarkisova S, Patrauchan M, Berglund D, Nivens D, Franklin M . Calcium-induced virulence factors associated with the extracellular matrix of mucoid Pseudomonas aeruginosa biofilms. J Bacteriol. 2005; 187(13):4327-37. PMC: 1151780. DOI: 10.1128/JB.187.13.4327-4337.2005. View

17.

Franklin M, Nivens D, Weadge J, Howell P . Biosynthesis of the Pseudomonas aeruginosa Extracellular Polysaccharides, Alginate, Pel, and Psl. Front Microbiol. 2011; 2:167. PMC: 3159412. DOI: 10.3389/fmicb.2011.00167. View

18.

Costes S, Daelemans D, Cho E, Dobbin Z, Pavlakis G, Lockett S . Automatic and quantitative measurement of protein-protein colocalization in live cells. Biophys J. 2004; 86(6):3993-4003. PMC: 1304300. DOI: 10.1529/biophysj.103.038422. View

19.

Hall-Stoodley L, Costerton J, Stoodley P . Bacterial biofilms: from the natural environment to infectious diseases. Nat Rev Microbiol. 2004; 2(2):95-108. DOI: 10.1038/nrmicro821. View

20.

Tischler A, Vanek M, Peterson N, Visick K . Calcium-Responsive Diguanylate Cyclase CasA Drives Cellulose-Dependent Biofilm Formation and Inhibits Motility in Vibrio fischeri. mBio. 2021; 12(6):e0257321. PMC: 8576532. DOI: 10.1128/mBio.02573-21. View